It's the eye shot of Akio as you can obviously tell she's crying. Because of her past. I'll tell you a little bit of her past.

She had one older sister and one younger brother. Their parents worked and so did the older sister (she gratuated high school early). The only problem in the family was their father. He was a drunk. One night he came home from the bar and from work. The kids were left at home alone while their mother was at work in fact it was almost time for her to come home. Every night when their father came home Akio and her older sister, Akari, would hide their brother, Tenshi, so their father couldn't find him because every night before their mother got home... Their father would make Akio and Akari have sex with him and each other. Finally when he came home this time he was in a drunk rage stage... They couldn't hide their brother in time... Their father killed both her brother and sister in front of her... *in the top right coner her memory of what they said, "Brother: "Dad...Daddy?" Sister: "Dad please... Don't... Daddy!" Father: shot them* Her mother came home and found their bodys *bottom left Mom: "Honey? Oh my god!" Akio: "Daddy no! Please... STOP!" Father: shot them both* Luckily it periced right through Akio's ribs and killed her mother. Akio faint though *bottom right Akio: "Dad..." Father: Shot himself* Later Akio returned home from the hospital alone even though Haku and Aku didn't want to leave her she insisted... *bottom center Akio: "Now I'm alone..."*

Sad isn't it?