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1 | | -This is *NOT* the official repository of SAMtools. The official SAMtools repository can be found at: http://samtools.sourceforge.net/ |
| 1 | +## This is *NOT* the official repository of SAMtools. |
| 2 | +The official SAMtools repository can be found at: http://samtools.sourceforge.net/ |
2 | 3 |
|
3 | | -Major fixes: |
4 | | -- added "samtools sample" command, to sample reads from a SAM/BAM file at a given frequency. |
5 | | -- added "samtools qa" command, to compute the mean and median coverage, as well a histogram from 1 to N (defined by param) containing the number of bases covered a maximum of 1X, 2X...NX. Furthermore, "other" information is also available in the output file, namely: |
6 | | - 1. Total number of reads |
7 | | - 2. Total number of duplicates found and ignored (duplicates are "found" based on the sam flag and are ignored in the counting of coverage) |
8 | | - 3. Percentage of unmapped reads |
9 | | - 4. Percentage of zero quality mappings |
10 | | - 5. Number of proper paired reads (based on sam flag of proper pairs) |
11 | | - 6. Percentage of proper pairs.e |
| 4 | +## Major fixes: |
| 5 | + - added "samtools sample" command, to sample reads from a SAM/BAM file at a given frequency. |
| 6 | + - added "samtools qa" command, to compute the mean and median coverage, as well a histogram from 1 to N (defined by param) containing the number of bases covered a maximum of 1X, 2X...NX. Furthermore, "other" information is also available in the output file, namely: |
| 7 | + - Total number of reads |
| 8 | + - Total number of duplicates found and ignored (duplicates are "found" based on the sam flag and are ignored in the counting of coverage) |
| 9 | + - Percentage of unmapped reads |
| 10 | + - Percentage of zero quality mappings |
| 11 | + - Number of proper paired reads (based on sam flag of proper pairs) |
| 12 | + - Percentage of proper pairs.e |
12 | 13 |
|
13 | | -Minor fixes: |
14 | | -- Check the write filehandle after opening for write. |
15 | | -- allow for user defined [lowercase] tags in header elements. |
16 | | -- allow the maximum memory for "samtools sort" to be specified with units. |
17 | | -- adjust for leading hard clip on colorspace reads. |
18 | | -- catches and reports an invalid BAM header, instead of segfaulting later on. |
19 | | -- fixes a small underflow/overflow bug in integer parsing. |
20 | | -- checks for a lowerbound in text entry box to avoid segfault in tview. |
| 14 | +## Minor fixes: |
| 15 | + - Check the write filehandle after opening for write. |
| 16 | + - allow for user defined [lowercase] tags in header elements. |
| 17 | + - allow the maximum memory for "samtools sort" to be specified with units. |
| 18 | + - adjust for leading hard clip on colorspace reads. |
| 19 | + - catches and reports an invalid BAM header, instead of segfaulting later on. |
| 20 | + - fixes a small underflow/overflow bug in integer parsing. |
| 21 | + - checks for a lowerbound in text entry box to avoid segfault in tview. |
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