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  • 5 commits
  • 40 files changed
  • 4 contributors

Commits on Jun 11, 2025

  1. feat: Add wrapper for Tandem Repeat Finder (TRF) utility (#4160)

    This PR provides support of executing TRF command line utility
    (https://prefix.dev/channels/bioconda/packages/trf) through Snakemake
    using Snakemake wrappers. On top of basic TRF feature(s) this wrapper
    additionally makes it easy for users to write Snakemake rules by
    providing following things:
    - In case user doesn't provide any parameters it automatically sets to
    recommended defaults by the TRF author as on the
    website(https://tandem.bu.edu/trf/help) and allows users to right a bare
    minimum Snakemake rule.
    - Raises errors or warning in case the parameter value or flag with
    options value is set to that not allowed by the author.
    - It filters or recognizes some mistakes and raise errors or warning
    based on that:
      - Error in case a known flag is mistyped like -m tp *m with feedback.
    - Warning and ignoring unknown flags if tried to entered like -) -Y etc.
    - Supporting multiple options for option value flag, -l20, -l 20 and
    -l=20.
    - In case user provides partial parameters list, it use recommended
    defaults with updates to those parameters which user provided
    specifically.
    - Handles missing log files gracefully.
    - Support to print the directory contents on Screen once output has been
    ready.
    - Has logging configured for debug, info mode etc.
    - Allows fasta format only as per snakemake wrapper utils:
    https://github.com/snakemake/snakemake-wrapper-utils/blob/master/snakemake_wrapper_utils/snakemake.py
    - Have basic integration tests in place with various rule scenarios. 
    - Updated meta for related documentation.  
    
    ### QC
    * [X] I confirm that I have followed the [documentation for contributing
    to
    `snakemake-wrappers`](https://snakemake-wrappers.readthedocs.io/en/stable/contributing.html).
    
    While the contributions guidelines are more extensive, please
    particularly ensure that:
    * [X] `test.py` was updated to call any added or updated example rules
    in a `Snakefile` -> _**yes, but in test-wrappers.py**_
    * [X] `input:` and `output:` file paths in the rules can be chosen
    arbitrarily
    * [X] wherever possible, command line arguments are inferred and set
    automatically (e.g. based on file extensions in `input:` or `output:`)
    * [ ] temporary files are either written to a unique hidden folder in
    the working directory, or (better) stored where the Python function
    `tempfile.gettempdir()` points to -> _**Not applicable in my case.**_
    * [X] the `meta.yaml` contains a link to the documentation of the
    respective tool or command under `url:`
    * [X] conda environments use a minimal amount of channels and packages,
    in recommended ordering
    
    
    <!-- This is an auto-generated comment: release notes by coderabbit.ai
    -->
    ## Summary by CodeRabbit
    
    - **New Features**
    - Added a Snakemake wrapper for the Tandem Repeat Finder (TRF) tool,
    enabling automated detection of tandem repeats in DNA sequences.
    - Provided environment configuration files for reproducible setup on
    Linux platforms.
    - Included metadata and documentation for wrapper usage, parameters, and
    outputs.
    
    - **Tests**
    - Introduced comprehensive test workflows and sample data to validate
    the TRF wrapper under various scenarios, including parameter variations
    and error handling.
    <!-- end of auto-generated comment: release notes by coderabbit.ai -->
    
    ---------
    
    Co-authored-by: rohan-ibn-tariq <[email protected]>
    rohan-ibn-tariq and rohan-ibn-tariq authored Jun 11, 2025
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  2. fix!: trimmomatic wrappers: remove parallel gzip, merge wrappers, add…

    …ed docs, and code refactoring. (#4158)
    
    <!-- Ensure that the PR title follows conventional commit style (<type>:
    <description>)-->
    <!-- Possible types are here:
    https://github.com/commitizen/conventional-commit-types/blob/master/index.json
    -->
    
    <!-- Add a description of your PR here-->
    Current parallel compression/decompression with `pigz` is no longer
    supported in latest versions of `Trimmomatic` and it breaks MultiQC
    reporting. Since it seems that the next version of Trimmomatic will have
    [parallel compression](
    
    usadellab/Trimmomatic@7220eba),
    this issues removes it, bumps versions, refactors code, merges the two
    PE/SE wrappers, and iproves docs.
    
    Closes #778 
    Closes #869 
    Fixes #961 
    
    ### QC
    <!-- Make sure that you can tick the boxes below. -->
    
    * [x] I confirm that I have followed the [documentation for contributing
    to
    `snakemake-wrappers`](https://snakemake-wrappers.readthedocs.io/en/stable/contributing.html).
    
    While the contributions guidelines are more extensive, please
    particularly ensure that:
    * [x] `test.py` was updated to call any added or updated example rules
    in a `Snakefile`
    * [x] `input:` and `output:` file paths in the rules can be chosen
    arbitrarily
    * [x] wherever possible, command line arguments are inferred and set
    automatically (e.g. based on file extensions in `input:` or `output:`)
    * [x] temporary files are either written to a unique hidden folder in
    the working directory, or (better) stored where the Python function
    `tempfile.gettempdir()` points to
    * [x] the `meta.yaml` contains a link to the documentation of the
    respective tool or command under `url:`
    * [x] conda environments use a minimal amount of channels and packages,
    in recommended ordering
    
    
    <!-- This is an auto-generated comment: release notes by coderabbit.ai
    -->
    ## Summary by CodeRabbit
    
    - **New Features**
    - Introduced unified configuration and metadata files for running
    Trimmomatic, supporting both paired-end and single-end workflows with
    various input/output formats.
    - Added a new wrapper script to streamline Trimmomatic execution within
    Snakemake workflows.
    
    - **Refactor**
    - Consolidated multiple environment and metadata files into a single set
    for easier management and reproducibility.
    - Unified Snakemake rules for Trimmomatic into a single, comprehensive
    Snakefile covering all compression and read-type combinations.
    - Removed legacy single-end and paired-end specific environment and
    metadata files along with deprecated wrapper scripts.
    
    - **Tests**
    - Simplified and consolidated test coverage for paired-end workflows;
    removed separate single-end tests.
    <!-- end of auto-generated comment: release notes by coderabbit.ai -->
    fgvieira authored Jun 11, 2025
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  3. fix: GATK variantrecalibrator wrapper path (#4126)

    <!-- Ensure that the PR title follows conventional commit style (<type>:
    <description>)-->
    <!-- Possible types are here:
    https://github.com/commitizen/conventional-commit-types/blob/master/index.json
    -->
    
    <!-- Add a description of your PR here-->
    Fixes #4125 
    
    ### QC
    <!-- Make sure that you can tick the boxes below. -->
    
    * [x] I confirm that I have followed the [documentation for contributing
    to
    `snakemake-wrappers`](https://snakemake-wrappers.readthedocs.io/en/stable/contributing.html).
    
    While the contributions guidelines are more extensive, please
    particularly ensure that:
    * [x] `test.py` was updated to call any added or updated example rules
    in a `Snakefile`
    * [x] `input:` and `output:` file paths in the rules can be chosen
    arbitrarily
    * [x] wherever possible, command line arguments are inferred and set
    automatically (e.g. based on file extensions in `input:` or `output:`)
    * [x] temporary files are either written to a unique hidden folder in
    the working directory, or (better) stored where the Python function
    `tempfile.gettempdir()` points to
    * [x] the `meta.yaml` contains a link to the documentation of the
    respective tool or command under `url:`
    * [x] conda environments use a minimal amount of channels and packages,
    in recommended ordering
    
    
    <!-- This is an auto-generated comment: release notes by coderabbit.ai
    -->
    ## Summary by CodeRabbit
    
    - **Chores**
    - Updated the source path for the wrapper used in the variant
    recalibration workflow.
    - Improved remote resource access by updating URLs and fetching methods
    in the variant recalibration tests.
    <!-- end of auto-generated comment: release notes by coderabbit.ai -->
    fgvieira authored Jun 11, 2025
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  4. feat: add param for mem_overhead (#4122)

    <!-- Ensure that the PR title follows conventional commit style (<type>:
    <description>)-->
    <!-- Possible types are here:
    https://github.com/commitizen/conventional-commit-types/blob/master/index.json
    -->
    
    <!-- Add a description of your PR here-->
    
    ### QC
    <!-- Make sure that you can tick the boxes below. -->
    
    * [x] I confirm that I have followed the [documentation for contributing
    to
    `snakemake-wrappers`](https://snakemake-wrappers.readthedocs.io/en/stable/contributing.html).
    
    While the contributions guidelines are more extensive, please
    particularly ensure that:
    * [x] `test.py` was updated to call any added or updated example rules
    in a `Snakefile`
    * [x] `input:` and `output:` file paths in the rules can be chosen
    arbitrarily
    * [x] wherever possible, command line arguments are inferred and set
    automatically (e.g. based on file extensions in `input:` or `output:`)
    * [x] temporary files are either written to a unique hidden folder in
    the working directory, or (better) stored where the Python function
    `tempfile.gettempdir()` points to
    * [x] the `meta.yaml` contains a link to the documentation of the
    respective tool or command under `url:`
    * [x] conda environments use a minimal amount of channels and packages,
    in recommended ordering
    
    
    <!-- This is an auto-generated comment: release notes by coderabbit.ai
    -->
    ## Summary by CodeRabbit
    
    - **New Features**
    - Introduced a configurable memory overhead setting to optimize memory
    allocation during FastQC runs.
    - Enhanced output descriptions for FastQC results, clarifying file
    formats and contents.
    - **Refactor**
    - Improved memory management during FastQC execution to optimize
    resource allocation per thread, enhancing performance stability without
    changing user workflows.
    <!-- end of auto-generated comment: release notes by coderabbit.ai -->
    fgvieira authored Jun 11, 2025
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  5. chore(master): release 7.0.0 (#4124)

    🤖 I have created a release *beep* *boop*
    ---
    
    
    ##
    [7.0.0](v6.2.0...v7.0.0)
    (2025-06-11)
    
    
    ### ⚠ BREAKING CHANGES
    
    * trimmomatic wrappers: remove parallel gzip, merge wrappers, added
    docs, and code refactoring.
    ([#4158](#4158))
    
    ### Features
    
    * add param for mem_overhead
    ([#4122](#4122))
    ([50f8acb](50f8acb))
    * add wrapper for MEGAHIT
    ([#4121](#4121))
    ([be71b0b](be71b0b))
    * Add wrapper for Tandem Repeat Finder (TRF) utility
    ([#4160](#4160))
    ([be31100](be31100))
    * enable gzip output for bedtools wrappers
    ([#3642](#3642))
    ([fc6df02](fc6df02))
    
    
    ### Bug Fixes
    
    * fix 4118
    ([#4119](#4119))
    ([00f7aaf](00f7aaf))
    * GATK variantrecalibrator wrapper path
    ([#4126](#4126))
    ([965df4d](965df4d))
    * remove hardcoded shrink type, and fix typos
    ([#4123](#4123))
    ([7832d9f](7832d9f))
    * trimmomatic wrappers: remove parallel gzip, merge wrappers, added
    docs, and code refactoring.
    ([#4158](#4158))
    ([c914d68](c914d68))
    
    
    ### Performance Improvements
    
    * autobump bio/bamtools/filter
    ([#4127](#4127))
    ([a5f375c](a5f375c))
    * autobump bio/bamtools/filter_json
    ([#4131](#4131))
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    ([#4130](#4130))
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    ---
    This PR was generated with [Release
    Please](https://github.com/googleapis/release-please). See
    [documentation](https://github.com/googleapis/release-please#release-please).
    snakemake-bot authored Jun 11, 2025
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