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Feulgen Stain Questions

The document discusses the Feulgen staining technique, which selectively stains DNA. It can be used to measure DNA concentration quantitatively using a microspectrophotometer. The Feulgen reaction relies on acid hydrolysis of DNA to produce aldehyde groups, which are then stained pink. Fixatives containing strong acids should be avoided, as they may pre-hydrolyze the tissue. The Feulgen staining technique was discovered by Robert Feulgen.

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Fatma Adel
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310 views5 pages

Feulgen Stain Questions

The document discusses the Feulgen staining technique, which selectively stains DNA. It can be used to measure DNA concentration quantitatively using a microspectrophotometer. The Feulgen reaction relies on acid hydrolysis of DNA to produce aldehyde groups, which are then stained pink. Fixatives containing strong acids should be avoided, as they may pre-hydrolyze the tissue. The Feulgen staining technique was discovered by Robert Feulgen.

Uploaded by

Fatma Adel
Copyright
© Attribution Non-Commercial (BY-NC)
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Complete the following questions 1 -The Feulgen technique selectively stains (DNA)

2 - It is possible to use an instrument known as a to actually measure the intensity of the pink feulgen reaction for a given organelle )microscpectrophotometer (

3 - The feulgen reaction is. Technique If the only aldehydes remaining in the cell are those produced from the hydrolysis of DNA

(a semi-quantitative)

4 - Acid hydrolysis resulting in and formation of . (the cleavage of the nitrogen bases , aldehyde groups) 5 - Sulphite rinses consist of.. )Potassium metabisulphite, Hydrochloric acid)

6 - Fixatives containing .should be avoided as this method depends on the acid hydrolysis of DNA, and acids in some fixatives may .the tissue

)Strong acids, pre-hydrolyze )

7- Feulgen stain is a staining technique discovered by

(Robert Feulgen )

PUT or X to the following questions


1- The staining intensity is proportional to the DNA concentration () 2- The Feulgen reaction can be carried out
after the use of any fixative (with the exception of Bouins fluid, which contains picric acid ()

3- The Feulgen reaction is used for the qualitative determination of DNA(X) Define following terms
1-Feulgen positive a specific cytochemical reaction to indicate the presence of DNA. It is based on converting DNA, but not RNA, to a purinic acid by acid hydrolysis with release of the aldehyde functions; the aldehyde functions then give a magenta color on treatment of the specimen with Schiff reagent. Tissue giving such a reaction is termed Feulgen positive

Choose the correct answer 1- Which of the following stains is used for routine histological examination? a. Wright's stain b. Hematoxylin and eosin stain c. Sudan stain d. Silver impregnation e. Masson's trichrome stain Answer: b

2- What color does hematoxylin stain structures? a. Orange b. Blue c. Pink d. Red e. Green Answer: b 3-Which of the following stain blue with H&E stain? a. Cytoplasm b. Collagen fibers c. Nucleus d. Elastic fibers e. Decalcified bone matrix Answer: c

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