OLYMPUS INVERTED RESEACH MICROSCOPE

INSTRUCTION MANUAL IMT

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OLYMPUS
Thi s inst ruct ion manual has been prepared f or the Oly mpus Invert ed Research Microscope Model
IMT. I t is recommended that you read the manual caref ull y in order to famil iar ize yourself f ull y
wi t h your microscope in order to obt ai n opt i mum performance f rom t hi s precision inst rument .
IMPORTANT
Observe the following points car efully:
Operation
1. A lways handle t he microscope wit h the care i t deserves, and avoid abrupt motions.
2. Avoid exposure of the microscope t o direct sunlight, dust and vi brati on.
3. Only use the tensi on adj ust ment ring for altering the t ension of th e coarse adj ust ment knobs.
Do not twist the two coarse adjustment knobs in th e op posite directi on s si mult aneously,
as th is causes damage.
4. Ascertain t hat t he voltage select or swi tc h on th e base plate is set t o conform wi t h t he l ocal
mains voltage.
5. Di sconn ect the li ne cord fr om the AC power out let for fu se repl acement .
6. Object ives provided to t he Mod el IMT are designed for observat ion of speci mens t hrough
cult ure vesse ls of 1mm th ickness. Therefore, be sure to use cult ure vessels, th e bottom of
wh ich is 1 mm O.3 t hick and evenly f lat .
Maintenance
1. Lenses must always be kept clean. Fine dust o n lesn surfaces sho uld be blown or wiped off
by means of a hand bl ower or a clean brush. Carefully wipe off oil o r fingerp rin t s deposited
on t he lens surf aces wi t h gauze moi stened with a small amount of xy l ene, al coh ol -et her
mixture (7 : 3) or et her.
2. Do not use organic sol utions t o wi pe t he surfaces of vari ous compon ents. Plast ic part s,
especi all y, should be cleaned wi t h a neut ral detergent.
3. Never di sassemble the mi croscope f or repair .
4. The microscope shoul d be st ored in it s contai ner immed iat ely aft er use. If this is not
possible, it shou ld be covered with the vi ny l dust cover provided . It is best to keep objectives
and eyepieces in desiccat ors. contai ni ng desiccants such as si li ca gel.
C.
CONTENTS
I. STANDARD EQUIPMENT
II . NOMENCLATURE
III. ASSEMBLY
IV. IDENTIFI CATION AN D FUNCTI ON OF VARIOUS COMPONENTS .
V. OPERATI ON . . . . . . . . . . . . . . . . . . . .
A. Electri c Equipment
1. Adj ustment of Mi nimum Vol tage
2. 30W Tungsten Bulb
B. Specimen Holders
1. With the Mechanical Stage
2. Without the Mechanical Stage
Observation Tubes . . . . . .
1. Interpupillary Distance and Diopter Adjustments
2. Eyepiece Cap
D. Illumination for Phase Contrast Microscopy
1. Ultra Long Working Distance I llumination
2. Long Working Distance I lluminat ion
E. Field and Aperture Iris Diaphragms
1. Field Iri s Diaphragm
2. Aperture Ir is Diaphragm
F. Focusing Adjustment . . . . . . . . . . . .
1. Tensi on Adjustment of Coarse Adjustment Knobs
2. Automati c Pre-focusing Lever
3. Adj ust ment of Stage Block Height
VI. OPTICAL DATA
VII. TROUBLESHOOTI NG
2
3
4
6
10
11
12
13
14
15
16
I. STANDARD EOUIPMENT
Model IMT
Component 201 203 211 213
Microscope stand with pillar and base IMTF 1 1 1 1
Revolving nosepiece IMTRE 1 1 1 1
Binocular tube BHBI 45 1 1 1 1
Plain stage with two extension plates IMTS 1 1 1 1
Me chanical stage with three specime n holders IMTMVR 1 1
Condenser holder IMTCH 1 1
Long working distance turret condenser IMT LWCD 1 1
For ult ra long work ing distance IMTULWD 1 1 1 1
Auxi liary lenses
For long worki ng di stance IMT LWD 1 1
Light annul us IMTRS-lO 1 1 1 1
Light annul i in
Light annulus IMTRS-20 1 1 1 1
mount IMTRS
Light annu lus IMT RS-40 1 1 1 1
Lamp house IMTLH 1 1 1 1
30W tungsten bulbs LS30 3 3 3 3
Ach.4X 1 1 1 1
Ach. l OX 1 1 1 1
Object ives
Ach. LWDC20X 1 1 1 1
Ach. LWDC Pl an 40X 1
,
Eyepieces BiCK10X, paired 1 1 1 1
Phase annulus slide IMTSL 1 1 1 1
Centering telescope CT 1 1 1 1
Spare fuses 100 -1 15A ( lAI or 220 -240V (0.8AI 2 2 2 2
Eyepiece caps 2 2 2 2
Green filter 45G-533 1 1 1 1
V inyl dust cover 1 1 1 1
Optional Accessories:
1. Trinocular Tube Model 8HTR45
2. Phot o Eyepieces FK2.5X, FK3.3X, FK5X, FK 6.7X
3. Photomicrographic System Camera Model PM 'O
4. Drawi ng Attachment Model 8 HDA
(Thi s att achment is provided with a magnif ication adjustment screw for use not only with
the Model IMT but al so wit h vari ous Olympus mi croscopes such as VANOX, BH, CH, etc.
For use with the Model IMT the adjustment screw on the drawing attachment should be set
to the posit ion "C".)
2
I I. NOMENCLATURE
The Model IMT is composed of var ious components and i nterchangeable accessor ies . A
var iet y of combi nations. standard or, opt io nal , is avail able acco rdi ng to you r requ irement s.
Lamp ho use
30W tungst en l amp
Plain st age
Mechanical st age
Attach able.
Eyepiece
Obser vation tu be
Phase annulus slide
Sta nd

Base
Auxi liary lens
For ultra long wor ki ng
dist ance.
3
t
Pill ar
Auxi liary lens
For long wo r ki ng
distance.
Long wo r ki ng d ist ance
tu rre t conde nse r
Condenser holder
Ob jecti ve
Revolving nosepi ece
Light an nuli for ultra
long worki ng distance
III. ASSEMBLY
The picture below i ll ust rat es th e sequent ial procedure of assembly . Th e number s ind icate the
assembly order of various components.
* Remove du st caps before mounti ng components. Take care to keep all gl ass surfaces clean and
avoid scrat chin g t he lens surfaces.
K nu rled ring
Pillar cl amping screw
<D Obj ect ive
<D Au xi liary len ses
(See NOTE 3.)
[Insert aux. le ns into th e dove-
tail mount of the suppo rt. I
@ Light

<l> Nosepiece
Clamp wit h sc rew provided .
o
3M t ungste n bul b
Tap ped
ho le
Lamp house
clamping
screw
f!
!
Aux i l iary lens
ci a mpi09 sc rew
E 5 = =
Lamp
house

@ Pillar I
(See NOTE 1.1 t
Stage mount -
iog __..::::::;
t
o
@ Specimen holder
Q) Condenser
St age
cl ampi ng 0
screws
Clamp st eadi ly JCo nde nser
wi t h a coin. holder

lv wit h a
coi n.
@ Plan st age
rt=='::;::;:=4
Stage bl ock lock i ng levers
Clamp steadily .
Lower limit st op pi n
Observation t ube
clampi ng screw
@]
1
I
@ Ph ase a nnulus slide
Insert t he phase annulus slide
into the slot in a manner t hat
its engraved surface wi t h fig-
ur es "1 0, 20 and 40" faces the
user.
@ Eyep iece
@ Eyepiece cap
@ Mechan ical stage
(See NOT E 2.1
Rotate t he ring t o stabilize t he base.
NOTE 1: The lower end of t he pill ar has two notches (l arger one at t he fr ont and smaller one
at t he back ) and a connect i ng cord comes out of it . Insert th e connect i ng cord and
l ower end of t he pi ll ar into th e base hol e vertically and clamp th e pill ar with screw at
t he bottom of t he base. The pillar direct ion is determi ned by f i tting t he notches so
that t he connect i ng cord passes through t he larger not ch and connects t o the
receptacle on the left hand side of the base back .
4
NOTE 2: Th e mechanical stage is attachable t o the right or left hand side of t he plai n st age and
clamped with a coi n. I f t he mechanical stage is attached, only one extension pl at e can
be attached to t he opposite side of t he plain stage.
@ How t o mount t he mechanical stage:
Fig. 1 shows t he mechanical stage attached on t he ri ght hand side of t he plai n stage. I t
is possibl e to attach the mechanic al stage on t he left side as shown i n Fig. 2 ac cord ing
t o your prefere nce. To correct ly attach t he mechan ical staqeon eit her side, al ign t he
edge of t he mechanical st age wi t h the plai n stage corner as indicated by arrow s.
Fi g. 1 Fig. 2
NOTE 3:
To t hose who wish to use a left-handed mechanical stage, t he mechanical stage with
left-hand low dr ive controls Model IMT MV L is op ti onall y avail abl e.
Set up the illuminat or:
a) To illuminat e f or l ong worki ng di st ance ( Fig. 31:
Mount t he aux il iary lens IMT-LWD CD t o t he lamp house suppor t and attach
the consenser and condenser holder t o t he plain st age; then adj ust the heigh t of t he
support , movi ng it ver t ically along t he pi l lar unt i l the lower edge of t he support
is aligned with t he yell ow l ine on t he pill ar and d amp.
Fig . 3 Fig , 4
b) To illuminat e f or ul tra long wor k i ng di stance ( Fig. 4):
Att ach t he auxi l iary lens IMT-ULMD CD to t he support @ and al ign th e l ower edge
of t he support t o t he red l i ne . For t his use, t he con denser and con denser hol der
should be removed.
cl For use of t he Abbe condenser BHeD or Achromatic/ aplanati ccondenser BHAAC:
It is necessary t o mount t he opti onal aux ili ary lens IMTA L t o t he lamp house
support and attach the condenser and con denser holder t o the stage. The lower
edge of t he suppor t should be aligned wi t h t he red l ine on t he pi l lar.
* For observat ion wi t h object ives 40x or higher , use an ordi nary obj ect ive and put
a cover glass under t he speci men.
d) For use of the contrast attachment BHNIC:
It is necessary to ali gn t he lower edge of t he support wit h the bl ack index li ne on
the pillar, and use t he auxi li ary lens IMT- AL.
* Only a cover gl ass of 0.17 mm t hick can be used.
5
IV. IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS
Filter mounts
I nser t the green fil t er 45G-533
in t o one of t he two mounts f or
phase co ntrast observat ion.
Fi eld ir is diaphragm lever
Interpupillary distance
scale
Mechani cal t ube lengt h
adjust ment rin gs
Tension adj ustment ri ng
Arrow mar k indicat es increase
in coarse adjustment t ension .
Coarse adj ustment knob /.-N__--- -
Pil ot lamp
Main swi tc h
6
Lamp house support
Support locki ng knob
Aperture i ris
d iaphragm lever
Condenser height
adj ustment kn ob
Low dri ve coaxial
cont ro l kn obs
Fi ne adjust ment knob
Graduated i n
increment s of 211 .
Ground t erminal
Sliding cont rol lever
As numbers increase. volt age i n-
creases.
I
Index li nes
Light annulus
centering screw
Condenser center ing
screw
Connect i ng cord
Level screw
Rota te t he ri ng t o
level t he base.
7
Stage cl ips
Stage ex tension p late
Phase annulus
insert io n slot
Nosepiece clamping
screw
L ine cord recept acle
II I
Aux i l i ary lens clampi ng scr ew
----1_-' ---------
a:::.:.:.nn:::::ulu::::....:s
111' Ligh t annul us cent er ing screws
Rheost at t rimmer screw
After sw itching on, if necessar y,
rotate this screw wi th a co in unt il
t he bulb is dimly l i t wi th the
slidi ng control lever at minimum
vo lta ge position.
Line voltage selector swit ch
Se t t he sw it c h t o conform with
line vol t age.
8
Fuse holder
Press wi t h a screwdri ver and ro-
tat e co unterclockwise t o remove
the ho lder.
SUMMARY OF PUTTING THE MICROSCOPE IN OPERATION
Model IMT
A. Mat ch t he li ne voltage selector swi tc h t o local mains voltage (page 8).
B. Switch on the light source.
C. Rotate t he trimmer screw until t he i lluminator dimly li ght s with sl idi ng cont r ol lever at
minimum voltage position (page 10 ).
D. Al i gn t he lamp house suppor t to t he index li ne on th e pi ll ar accord i ng t o your micr oscopic
purpose (page 5).
E. Place a specime n on th e stage (page 10 ).
F. Coarse focus with th e lOX object ive.
* If necessary, stop down the aperture diaphra gm to r easier focusing.
For ph ase co ntrast , ho wever, make it a po int of opening the full ape rt ure.
G. Make interpupi llary and di opter adju stments (page 11 ).
H. Ad just condenser height if t he long working di st ance conde nse r IMT*LWCD is used
(page 13) .
I. Engage the pha se annulus " 10" .
J . Engage t he light annulus " 10".
* For th e use of th e auxili ary lens IMT ULWD, engage th e light annulus RSl 0.
K. Center t he light annulus by means of th e centering telescope CT (page 12).
L. Swing the obj ecti ve of desired magnificat ion into light path .
M. Match the light annulus to t he magn ificat ion of objective in use and center t he annulus.
N. Adjust light int ensity.
O. Fine focus.
P. Adjust aperture diaphragm and field diaphragm (page 13).
(Cut off t his page at dotted li ne and pu t it on th e wa l l near
th e mi crosc ope to see as a remi nder of micr oscopic procedu re.l
9
V. OPERATION
A. Electric Equi pment
1. Adju stmen t of Minimum V ol t age
The minimum voltage req u ir ed fo r t he ligh t sour ce can be adj ust ed w i t h t he rh eost at
tr immer screw at th e mi croscope base plat e in accordance with t he li ne voltage and
f requency . Th e bu il t- i n rheostat incorporates a thyr ist or i n i ts semiconductor c i rcu it for
the following advantages :
a) Ext remel y fi ne adj ustment of l igh t intensity.
b) El imination of f l ick er and stabi l izat ion of l igh t
int ensi t y .
c) Increased l if e expect ancy of the bul b.
For adjustment of t he minimum li ne vo lt age, ascer-
tain that the voltage selector swi tch is set to con form
with t he local mains volt aqe, and t he slidi ng co ntrol
lever is positi oned closest to you (l ow vo! taqcl ,
and then activate the mai n switch CD . I f th e bu lb is Fig. 5
di mly lit, t he seconda ry vol t age is cor rect. I f it is
not l it at al l, rot at e t he rheost at t r i mmer screw gradual ly wit h a co i n, unti l t he bulb is
di mly l it ; t hen push t he sl id ing cont ro l lever fo rwa rd in order t o obtain opt imum li ght
inte nsit y. {Fig. 51
2. 30W Tun gst en Bulb
The standard li ght source i ncorporates a 30W pre-centered tu ngst en fil ament bul b,
pr ovided wi t h a socket for pos it ive cont act , el iminati ng t he problems of defect ive contact
and over -heat i ng. When used at t he sett ing " 6" on t he control lever scale, th e average
l ife of t he bu lb LS30 is lon ger t han 200 hour s. Thi s is, however, greatly reduced, if t he
bulb is used at higher voltage. Theref or e, it is advi sable t o avoid prolonged use at readi ngs
over "6" (in t he red zone).
* It is recommended t o avoid swi t ching on and off the t ungsten bulb wit h t he sl idi ng
control lever at high i nt ensit y position (away fr om the user ). It reduces bulb life.
B. Specimen Hol ders
1. With t he Mechani cal Stage
Make a cho ice of specimen holder s accordi ng t o your speci men and place i t on t he stage.
Stage plate holder 60mm d ia.
Petr i dish holder
SI ide holder
2. Wit hout th e Mechanic al Stage
Place a speci men on the plai n stage di rectl y and move it manuall y .
@ Thi ck ness of cul t ure vessel s
Objectives provided wit h t he Mo del IMT ar e designed t o observe specimens t hrough 1 mm
t hick glass. Make it pract ice, theref ore, t o use culture vessels or slides of thi ckn ess
1 mmO.3.
10
@ Use of th e corr ect ion collar of th e obj ect ive LWDC Plan 40X
After coarse and fine adj ust ments, rotate t he correct ion col lar . keeping t he specimen in
fine focus until opt i mum resoluti on is obtai ned. A proper use of th is coll ar is specia lly
ef fecti ve t o prevent the deteri ora tion o f t he objective resol uti on to be caused by t he un -
even th icknesses of various pet ri dishes, cult ure bottl es, etc .
C. Observat i on Tubes
1. Interpu pill ary Dist ance and Diop t er Adjustments
1) Hol d the knurl ed sli di ng grips <D of th e ri ght
and left eyepiece tubes wit h both hands and push
t he t ubes toget her or pull th em apart lat erall y ,
wh ichever is required, while looking t hroug h th e
eyepi eces wit h bot h eyes, until perfect binocular
vi son is ob tai ned. (F ig. 6)
2) Rotate the tube length adju stment ring on t he
right eyepiece tu be t o match your interp up i l lary
distance setti ng whci h yo u obtai ned from th e
scale .
Fig. 6
31 Look at t he i mage through the right eyepiece with your ri ght eye and focus on t he
specimen wi th t he coarse and f ine adju stment knobs.
4) Next, lookin g at t he image through the left eyepi ece with your left eye , rotate t he
tube length adjustment ring @ t o f ocus on the speci men wi t hout using th e coarse
and fi ne adj ust ment knobs.
2. Eyepi ece Cap
The eyep iece cap is recommended f or t hose who wear eyeglasses. It prevent s scrat ching
t he eyeglasses.
D. Ill uminat ion f or Phase Cont rast Microscopy
1. Ult ra Long Work i ng Di st ance Il l uminat ion
The t otal work i ng di stan ce is 55mm. long enough
to permit observation of large size culture bott les.
1) Move the phase annu lus sli de CD unt i l the rnaq-
nif icat ion i ndex engraved on th e slide matches
th e ob ject ive magni fica tion i n use (F ig. 7) .
* Make sure th at the sli de cl ick s int o position .
2) Place a specimen on th e stage and bring it into
focus.
NOTE: It is suggest ed t o stop down the field i ri s
idaphragm for easier fo cusing . ( Fig. 8)
After f ocusing, op en th e full aper ture @ .
3) Insert the li ght annul us into th e auxiliary lens.
NOTE: Use t he l ight annul us t hat mat ches the
object ive magnifi cat ion.
11
Fig. 7
Fig. 8
- - -- - ---- - -
4) Remo ve one of the eyepi eces from t he observat ion t ubes and insert the cent er ing
telescope int o t he observat ion tube .
5) Rotate t he top lens assembly of t he center ing telescope to bri ng the br ight r i ng ( l ight
annul us) and the dar k r ing (phase annulus) int o focus.
6) Operate the center i ng screws of the li ght annulus unti l both annul i are concentric
and superi mposed. (Fig. 9)
Fi g. 9
7) Remove t he cent er i ng t elescope and insert t he eyepiece bac k into t he observat ion
tube. Now you can st art you r phase cont rast observatio n.
NOTE: 1. Repeat the procedure t hrough 1) to 6) above each t ime objectives are
changed.
2. In case of ul t ra long worki ng dist ance ill uminat ion, t he fi eld i r is diaphragm
@ in Fig. 8 is used as apert ure diaphragm.
2. Long Wor king Dist ance Illum inat ion
The total wo r ki ng dist ance is 20 mm so long as t o per mit observation of large size Petri
dishes.
1) Set th e tu rret condenser I MT-LWCD at the " 0" posi ti on.
2) Coarse f ocus with the 10 X object ive.
NOTE: It is suggest ed t o stop down sli ghtly t he apert ure i ri s dia phragm @ fo r easier
f ocusing (F ig. 10)
3) St op down the f ield i r is diaphragm t o the mini mum by means of the lever CD . Rotat e
t he condenser height adjustment kn ob unt i l t he i mage of t he f ield d iaphragm
is visibl e sharply in the field of view.
Fi g. 10
12
4) Br ing the image of the f ield diaphragm int o the center of the f iel d by means of the
condenser centeri ng screw (Fi g. 10) . Re-open the d iaph ragm until the small r ing
of the di aphragm becomes a larger pol ygonal r ing around the ci rcular edge of the f ield
(Fi g. 11).
Fig. 11
5) For phase cont rast observat ion, open t he apert ure di aphragm fu ll y .
6) Rotate the turret condenser until the light annulus " ' 0" is in posit ion.
7) Match the phase annulus sl ide at the " 10" positi on (F ig. 7).
8) Insert the centering telescope CT into the observation tube after removal of the eve-
piece. and rotate the top lens assembly of the CT to bring the l ight annulus and phase
annulus into focus.
9) Operat e the center i ng screws of the li ght annulus (Fig. 10) until l ight annulus and
phase annulus are concentric and superimposed.
* After complete cent rat ion, clamp the centering screws with care.
10) Remove the CT and insert the eyepiece back into the observation tube. Now it is
ready for phase contrast observation .
NOTE : Mat ch the light annulus and phase annul us to the objecti ve magnif icat ion
whenever object ives are changed, but re-centr ati on is not necessary.
E. Field and Apert ure Iris Diaphragms
1. Field Iris Diaphr agm
The field iris diaphr agm controls the diameter of the ray bundle impinging on the speci-
men surface and thus increases image defin ition and reduces glare.
2. Aperture Iri s Diaphragm
In order to achieve optimum obj ecti ve perf ormance in brightfield. the opening of the
aperture iris diaphragm should be mateched to t he numerical aperture of the object ive in
use. I t is often preferable, however, t o stop down the aperture diaphragm by about 70%
to BO% of the object ive N.A. (Fig. 121.
During phase contrast microscopy. however. the aperture iris diaphragm should be kept at
fu ll ope ni ng.
70 -80%
30 - 20%
Fig. 12
13
F. Focusing Adjustment
1. Tension Adjustment of Coarse Adjustment Knobs
A t ension adjust ment ring CD is provided next t o t he
right hand coarse adjust ment knob. With thi s device
t he tension of t he coarse adj ustment is freely adj usta-
ble for either heavy or l ight movemen t , dependi ng on
operator preference (F ig. 13l. However , do not
loosen t he tension adjust ment r ing too mu ch, because
the stage drops or t he f i ne adjustment knobs sl ip
easily . I f i t recommended to tig hten t he r i ng especial -
ly f or prolonged observat io n or cincphot ornlcro-
qrap hv .
NOTE: Be caref ul not t o rota te t he right and lef t
coarse adjust ment knobs i n t he opposite
di rect ions si mul taneously.
2. Automati c Pre-focusing Lever
Th is lever CD is provided to prevent possible cont act
bet ween specimen and obj ecti ve as well as to simpl if y
coar se f ocusing. The lever is locked after coarse focus
has been accomplished. This prevents f urt her down-
ward t ravel of the stage by means of t he coarse
adjust ment knobs and automat ical ly provides a li mit -
ing stop if the stage is raised and t hen lowered again.
The automat ic prefo cusi ng lever does not restri ct f ine
foc using (Fig. 141.
Fig. 13
Fig. 14
3. Adjustment of St age Block Height
The stage bl ock shou ld be al igned wi t h th e stage bi ock height index " B" , engraved on
the microscope stand (Fig. 14) for bi ological and tissue culture observat ion.
Beside the vert ical movement of t he stage by means of coarse and fine adj ust ments, th e
stage block hei ght can be changed upwa rd af t er loosening two stage block loc k i ng levers
f rom the " B" index to th e "NIC" i ndex (f or t he di fferenti al i nt erference co ntrast attach-
ment) or to t he " RFL" ind ex (f or the fluorescence vert ical ill um i nator) .
On the ot her hand, after removing the lower limi t stop pin, it is also possi ble to l ower t he
stage from t he " B" i ndex for observation of a specimen with larger dept h wit hi n t he
working distance of t he object ive in use.
CAUTION: After lowering the st age block from the " 6" index, however, be
careful not to hit the objective front lens against the stage by rotating the
nosepiece. Make it a point to hold the st age block steady with your hands
while you change the stage block height when the lower limit st op pin is re-
mo ved .
14
VI . OPTICAL DATA
Objective Type Achromat Plan Achromat
Magnifi cat ion 4X l OX LWO- LWO-CPlan 40 X
C20 X
NA 0. 10 0.25 0.40 0.55
W.O. (rnm) 18.77 6.78 5.44 2.00
Focal length (mm) 28.45 16.08 7.79 4.36
Resolving power ( Il) 3.4 1.3 0.84 0.6 1
Remarks Spring-loaded,
Eyepi ece cor rect ion col lar
BiCKlOX Total magnifi cati on 40X 100X 200X 400X
(Fi eld
Focal depth ( Il) 172.5 27.6
~ 1 9
3.87
number
17.2)
Field of vi ew (mm)
@ ) (1.:;'2> _
1 8 ~
0.43
---
(The resolving power is obtained at the fully opened aperture d iap hraqrn.]
Techn ical terms:
Working distance .
Numer ical aperture
Resolving power .
Focal depth
Field number .
Field of view di ameter .
The di stance from the specimen slide or culture vessel
to the nearest point of the objective.
The N.A. represents a performance number wh ich could
be compared to the relati ve aperture of a camera lens.
The quantity of light which the objective receives f rom
the object increases with the square of the performance
number .
The resolving power of a lens is measured by its abili ty
to separate t wo points.
The distance between the upper and lower limits of
sharpness in the image formed by an opt ical system. As
you stop down the aperture iris diaphragm. the focal
dept h becomes larger . The larger t he N.A. of an objec-
t ive the shallower the f ocal dept h.
A number that represents the diameter in mm of the
image of t he f ield diaphr agm that is formed by the lens
in front of it.
The actual size of the fi eld of view in mm.
15
VII. TROUBLESHOOTING
Troubles Causes Remedi es
1. Opt ical Syste m
a) Field of view is cut off or Auxiliary lens is not correctl y Correct ly mount t he lens. (Page 5)
il luminat ed irregularly _ mounted.
Lamp house support is not posi- Ad j ust t he height co rrect ly. (Page
tioned at correct height . 5)
Nosepiece does not click in posi- Sl ightly rotate the nosepiece unt il
tion. it clicks into posit ion.
Illuminat ion is not correct ly match- Correct it referring to " I I I. AS-
ed to the observation purpose. SEMB LY" at page 5.
Condenser is not correct ly mounted Remount the condenser correct ly.
on the holder.
Field diaphragm is stopped down Open the diaphragm properl y . (Page
excessively . 13)
Bulb is not correctly att ached. Re-insert it into t he socket all t he
way .
Phase annulus slide i s not click Cli ck it into position.
stopped in posit ion.
b) Dust or dirt is visible in Dust on condenser top lens.
the field of view.
Dirty speci me n. Remove dust .
Dust on eyepiece.
c) Excessive image contrast. Condenser is excessively in high Lower the condenser.
position.
Apert ure ir is diaphragm is stopped Open the diaphragm. (Page 13)
down excessively.
d) Resolutio n problems: Object ive is not correct ly posit ion- Slightly rotate the nosepi ece unt il
ed i n l ight path. it cl ick s into posit ion.

I mage is not sharp.

I nsuffici ent cont rast. I n case of LWDC Pl an 40X, cor- Adj ust the collar correctl y. (Page

I mage detai ls lack ce- rect lon colla r is not adj ust ed. 11)
f init ion.
Dust on object ive front lens.
Dirty specimen. Clean.
Dust on condenser lens.
I ll uminat ion is not mat ched to ob- Cor rect the ill uminat ion ref erring
servat ion pu rpose. to " I I I. ASSEMBLY" at page 5.
Speci men sl ide is mounted on the Reverse the specimen sli de. (Page
stage upside down. 10)
16
Troubles Causes Remedies
e) Specimen image is part ial- Objective is not correctly position- Slightly rotate the nosepiece unti l
Iy out of f ocus. ed i n li ght path . it clicks into position.
Specimen is not correctly posit ion- Piece the specimen correct ly on the
ed on the stage.
st age. (Page 10)
f ) When obj ectives are chang- Mechanical tube lengt h is not cor- Adjust t he lengt h with the tube
ed they are not par fo cal . rectlyadjusted. length adjustment rings on observa-
t ion t ube. (Page 11)
gl No effecti ve phase con- Aperture iris diaphragm is stopped Open.
trast isobtained. down.
Object ive, l ight annulus and phase Mat ch t hem cor rect ly . (Page 12)
annulus are not correctly matched.
Light annulus and phase annulus Center them correc t ly. (Page 12)
are not correctly centered.
2. Electric System
a) Illuminator is to o br ight Vol t age selector switch is not Match the selector switch to mains
(or too dark). matched to mains vol tage. voltage.
Mains voltage is too high (or t oo Adjust mainsvoltage with a variable
low). vo! tage transformer.
Rheostat trimmer screw is not Adjust t rimmer screw unt il the
matched to mains voltage. bulb is diml y lit with sliding con-
trol lever positi oned at minimum
vol tage. (Page 10)
b) Vo l tage f or illuminator Voltage selector switch i s not Match the selector switch to mains
cannot be regulated. matched to mains voltage. voltage.
Mains vol tage is too low (or too Adj ust mains voltage wit h the varl -
high).
able voltage transformer.
cl
The ligh t f l ickers and the Mains voltage is unstable. Use a voltage stabilizer.
intensity is unstable.
The f il ament of bulb is l i kely t o Replace the bu lb.
burn out.
Loose electric connect ion. T ighten the connect ion.
dl Fuse burns out too often. Fuse is not a standard one. Use a standard fuse.
Voltage selector switch is not Match the switch to mains voltage.
matched to mains voltage.
e) Pil ot lamp l ights bu t iI- Th e bulb is burned out. Repl ace the bu lb.
luminator bulb does not.
Loose electr ic connection. T ighten the connection.
f ) Reduced bu lb l if e. Vo l tage selector switch is not Match the selector switch to mains
matched to mains voltage. voltage.
The bulb is not a standard one. Use a st andard bu lb.
17
Troubles Causes Remedies
Bul b is used on higher voltage to Keep t he output voltage under 6V
increase light intensity. (wi th th e sli di ng cont rol lever at
"6" setting on the scale) as much as
possible, or usea high intensity light
source such as a halogen lamp, etc.
3. Focusing
a) Coarse adjustment is too Tension adj ustment ring is t ighten- Loosen the tension adjustment ring
t ight. ed too much. properly. (Page 14)
The user is trying to lower the stage Unlock the pre-focusing lever. (Page
passing over the lower focusing 14)
limit imposed by the engaged pre-
focusing lever.
b) Stage drops and specimen The tension adjustment ring is too Ti ghten t he ring pr operly. (Paqe 14)
goesout of focus. loose.
c) Stage cannot be lowered Pre-focusing lever is engaged i n Unlock the lever . (Page 14)
to the lower limi t of the higher than focusing position.
work ingrange.
d) St age cannot be raised to St age bl ock is not aligned to the Adjust the stage bl ock height cor-
the upper limit . correct stage block height index. rect lv. (Page 14)
4. Observation Tube
a) Incomplete binocular vi- Interpupillary distance is not cor- Correct the interpupil lary distance.
sion. rectly adjust ed. (Page 11)
Diopt er adjustment is incomplete. Complet e diopter adju st ment. (Page
11)
The user is unaccustomed to bino- Prior to looking at specimen details,
Gular vision.
t ry to look at the ent ire field of
view, or look at a far away object
before resuming microscopic obser -
vation.
5. St age
a) Image easily goes out of The stage is not correctly clamped. Clamp the st age securely.
focus when you touch the
stage.
b] The specimen st ops mid- Specimen is not correct ly posi- Adjust specimen positi on.
way on t he east-west tioned on stage.
traverse.
c) Specimen stops midway on Mechanical stage is not mount ed in Mount the mechanical stage in cor-
the north-south traverse. correct positi on. rect position. (Page 5)
1B
MEMO : .
19
OLYMPUS OPTICAL CO. LTD. 01VMPLJS
..:::tOKYo:..
43-2, HATAGAYA 2-CHOME,SHIBUYA-KU
TOKYO, JAPAN.
Prited in Japan 7904 M03