Food Testing

Food Safety and Quality Diagnostics

Bio-Rad Solutions

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Contents
PART 1 • Routine Testing - Quality Indicator 3
Preparation of dilutions 3 Coliforms - ISO standards 6
Total flora 3 Coliforms - Alternative method: RAPID’E.coli 2 Agar 7
Escherichia coli - ISO standards 4 Enterobacteriaceae 8
Escherichia coli - Alternative method: Enterobacteriaceae - Alternative method:
RAPID’E.coli 2 Agar 5 RAPID’Enterobacteriaceae 9

PART 2 • Pathogen Testing 10

Salmonella - ISO standard 10 Listeria spp. - Alternative method:
iQ-Check Listeria spp. real-time PCR Kit 22
FDA-BAM Salmonella protocol 11
Escherichia coli O157:H7 - Alternative method:
FSIS Salmonella MLG 4.07 11
RAPID’E.coli O157:H7 Agar 23
Salmonella - Alternative method:
STEC - iQ-Check STEC VirX and SerO
RAPID’Salmonella Agar double enrichment protocol 12
real-time PCR Kits 24
Salmonella - Alternative method:
Escherichia coli O157:H7 - Alternative method:
RAPID’Salmonella Agar short protocol 12
iQ-Check E.coli O157:H7 real-time PCR Kit 24
Salmonella - Alternative method:
Campylobacter spp. - ISO standard 25
iQ-Check™ Salmonella II real-time PCR Kit 12
FDA-BAM Campylobacter spp. protocol 26
Cronobacter spp. - ISO standard 14
Campylobacter spp. - Alternative method:
FDA-BAM Cronobacter spp. 14
iQ-Check Campylobacter real-time PCR Kit 26
Cronobacter spp. - Alternative method:
Campylobacter spp. - Alternative method:
RAPID’Sakazakii Agar short protocol 15
RAPID’Campylobacter spp. 27
Cronobacter spp. - Alternative method:
Staphylococcus aureus - ISO standards 28
iQ-Check Cronobacter spp. real-time PCR Kit 15
FDA-BAM Staphylococcus aureus 29
Listeria monocytogenes - ISO standards 16
Staphylococcus aureus - Alternative method:
FDA-BAM Listeria monocytogenes protocol 18
RAPID’Staph Agar 29
Health Canada Listeria monocytogenes
Sulfite-reducing bacteria 30
protocol MFHPB-30 18
Clostridium perfringens 30
Listeria monocytogenes - Alternative method:
RAPID’L.mono Agar 19 Bacillus cereus 31
Listeria monocytogenes - Alternative method: Shigella 31
iQ-Check Listeria monocytogenes II real-time PCR Kit 20
Validations 32
Listeria spp. - Alternative method: RAPID’L.mono Agar 21
Range for Food Testing 33
Listeria spp. - Alternative method:
RAPID’Listeria spp. Agar 21

This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products.
It is not a contractual document.

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Routine Testing • Quality Indicator

Preparation of dilutions Total flora

NF EN ISO 6887-1 (09/1999) NF EN ISO 4833 (05/2003)

Preparation of test samples, initial General guidance for the

suspension, and decimal dilutions enumeration of microorganisms
for microbiological examination
PCA Bacteria
Part 1: General rules for Colony-count technique at 30°C
preparation of dilutions for
Ordering Information microbiological examination
Catalog # Description

Buffered Peptone Water/Broth m g or v ml of sample Initial suspension

Ready-to-use + and/or
355-4179 225 ml x 6 bottles 9 x m g or 9 x v ml of decimal dilutions
355-5795 3 L x 4 bags Tryptone Salt or Buffered
355-5790 5 L x 2 bags Peptone Water
Dehydrated (and following dilutions)
356-4684 500 g

PCA (Plate Count Agar)/Agar

Ready-to-use PCA
355-4459 100 ml x 6 bottles
355-4457 200 ml x 6 bottles
356-3989 90 mm x 20 dishes

Dehydrated
Dilution 10-1
356-4475 500 g

Tryptone-Salt/Diluent
Ready-to-use 30°C
355-5754 9 ml x 25 tubes 72 hrs
355-5756 90 ml x 6 bottles
355-5796 3 L x 4 bags

Dehydrated
356-4544 500 g
(and following dilutions) Enumeration

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Escherichia coli - ISO standards

Ordering Information
Catalog # Description ISO 16649-1 (04/2001) ISO 16649-2 (04/2001)

Buffered Peptone Water/Broth Horizontal method for the enumeration Horizontal method for the enumeration
Ready-to-use of β-glucuronidase-positive E. coli of β-glucuronidase-positive E. coli
355-4179 225 ml x 6 bottles
355-5795 3 L x 4 bags Part 1: Colony-count technique at Part 2: Colony-count technique at
355-5790 5 L x 2 bags
44°C using membranes and 44°C using 5-bromo-4-chloro-
Dehydrated 5-bromo-4-chloro-3-indolyl 3-indolyl -D-glucuronide
356-4684 500 g -D-glucuronide

EC Medium/Broth
Dehydrated Sample/ Sample/
356-4764 500 g initial suspension initial suspension
Kovacs Reagent
(for Indole detection) Dilutions Dilutions
355-5313 15 ml x 2 bottles

Peptone Water (Indole Free)/Broth

Ready-to-use MMGA with membrane TBX
355-4180 9 ml x 25 tubes
Room
Dehydrated Resuscitation t° 15 min
356-4334 500 g (2 X)*
and 37°C
RAPID’E.coli 2/Agar 4 hrs ± 1 hr
Ready-to-use
355-5297 200 ml x 6 bottles TBX
355-5299 100 ml x 6 bottles 44°C
18-24 hrs
Dehydrated
356-4024 500 g

TBX/Agar Direct enumeration

Ready-to-use
355-5309 100 ml x 6 bottles 44°C
355-5307 200 ml x 6 bottles 18-24 hrs

Dehydrated
356-4035 500 g Direct enumeration
Tryptose Lauryl Sulfate/Broth
Dehydrated
356-4774 500 g

Tryptone-Salt/Diluent
Ready-to-use
355-5754 9 ml x 25 tubes
355-5756 90 ml x 6 bottles
355-5796 3 L x 4 bags

Dehydrated
356-4544 500 g * Please refer to ISO 7218: 2007

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Routine Testing • Quality Indicator

Escherichia coli
Alternative method:
RAPID’E.coli 2 Agar

ISO/TS 16649-3 (09/2005) ISO 7251 (07/2005) Enumeration of E. coli

NF VALIDATION according to ISO 16140
Horizontal method for the enumeration General guidance for detection N° BRD 07/1-07/93 and BRD 07/7-12/04
of β-glucuronidase-positive E. coli and enumeration AOAC-RI approved N° 050601**
of presumptive E. coli NordVal approved N° 020
Part 3: Most probable number Health Canada (Pending)
technique using 5-bromo-4-chloro- Most probable number technique All food stuffs
3-indolyl -D-glucuronide

Sample/initial suspension Sample/ Sample/

initial suspension initial suspension

Dilutions
Add 10 ml in And subsequent dilution
3 tubes of double Add 1 ml in 3 tubes
strength MMG of single strength MMG Tryptose Lauryl
Sulfate RAPID’E.coli 2
37°C
24 hrs ± 2 hrs

Single Double
concentration (or)* concentration

44°C/37°C
Subculture each tube presenting 18-24 hrs
yellow coloration on a dish of TBX 37°C 37°C
24-48 hrs 24-48 hrs
44°C ± 2 hrs ± 2 hrs Direct enumeration
20-24 hrs
EC Medium of typical colonies*
44°C
24-48 hrs
± 2 hrs

Each tube showing the presence of Peptone Water

blue colonies on TBX
should be considered positive 44°C
48 hrs
± 2 hrs

Indole test reading and calculation

Calculate the most probable number * Enumeration of typical colonies for E. coli
at 44°C; for E. coli and coliforms at 37°C
from the number of positive tubes ** Please refer to AOAC certificate for
at each dilution * For detection method only validated matrices

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Coliforms - ISO standards

ISO 4831 (08/2006)

Microbiology of food and animal feeding stuffs

Horizontal method for the detection
and enumeration of coliforms
VRBL Coliforms
MPN technique

Ordering Information
Catalog # Description
Sample
BGBLB (Brilliant Green Bile
Lactose Broth)/Broth
Ready-to-use Dilutions
357-8024 10 ml x 25 tubes

Buffered Peptone Water/Broth

Ready-to-use Tryptose Lauryl Sulfate
355-4179 225 ml x 6 bottles
355-5795 3 L x 4 bags
355-5790 5 L x 2 bags

Dehydrated
356-4684 500 g Single Double
RAPID’E.coli 2/Agar
concentration (or)* concentration
Ready-to-use
355-5297 200 ml x 6 bottles
355-5299 100 ml x 6 bottles 30°C or 37°C 30°C or 37°C
24-48 hrs ± 2 hrs 24 hrs ± 2 hrs
Dehydrated
356-4024 500 g

Tryptose Lauryl Sulfate/Broth

Dehydrated
356-4774 500 g
BGBLB
Tryptone-Salt/Diluent
Ready-to-use
355-5754 9 ml x 25 tubes 30-37°C
355-5756 90 ml x 6 bottles 24 hrs (+ 24 hrs)
355-5796 3 L x 4 bags

Dehydrated
356-4544 500 g Reading (gas +)
VRBL (Violet Red Bile Lactose)/
Agar
Ready-to-use
355-4799 100 ml x 6 bottles

Dehydrated * For detection method only

356-4594 500 g

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Routine Testing • Quality Indicator

Coliforms
Alternative method:
RAPID’E.coli 2 Agar

ISO 4832 (02/2006) Enumeration of coliforms

NF VALIDATION according to ISO 16140
Microbiology of food and animal N° BRD 07/8-12/04
feeding stuffs AOAC-RI approved N° 050601**
Horizontal method NordVal approved N° 020
for the enumeration of coliforms Heath Canada (Pending) RAPID’E.coli 2 E. coli Other
All food stuffs Coliforms
Colony-count technique Total Coliforms

Sample/ Sample/
initial suspension initial suspension

Dilutions Dilutions

VRBL RAPID’E.coli 2

(2 X)*

30-37°C 37°C
24 hrs 18-24 hrs
± 2 hrs

Enumeration Direct enumeration

of characteristic colonies of typical colonies*
and confirmation
for atypical colonies

Coliforms

• Diameter ≥ 0.5 mm
• Purple with or without a red
zone of precipitated bile * Enumeration of typical colonies for E. coli
at 44°C; for E. coli and coliforms at 37°C
** Please refer to AOAC certificate for
* Please refer to ISO 7218: 2007 validated matrices

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Enterobacteriaceae

ISO 21528-1 (08/2004)

Microbiology of food and animal feeding stuffs

Horizontal methods for the detection
and enumeration of Enterobacteriaceae
VRBG Enterobacteriaceae
Part 1: Detection and enumeration by MPN technique
with pre-enrichment
Ordering Information
Catalog # Description
Sample
Buffered Peptone Water/Broth
Ready-to-use Non-selective
355-4179 225 ml x 6 bottles pre-enrichment
355-5795 3 L x 4 bags
355-5790 5 L x 2 bags Buffered Peptone Water
37°C
Dehydrated
18 hrs ± 2 hrs
356-4684 500 g

EE/Broth EE
Dehydrated
Selective 37°C
356-4794 500 g
enrichment 24 hrs ± 2 hrs
Nutrient (2.8%)/Agar
Ready-to-use VRBG
355-4478 8 ml x 25 tubes

Dehydrated 37°C
356-4484 500 g 24 hrs ± 2 hrs

Oxidase Test
355-3834 2 x vials of
50 oxidase disks

VRBG (Violet Red Bile Glucose)/ Confirmation

Agar Nutrient (2.8%)
Ready-to-use
37°C
355-4239 100 ml x 6 bottles
24 hrs ± 2 hrs
Dehydrated
356-4584 500 g
Biochemical confirmation

Oxidase Dextrose BCP

37°C
24 hrs ± 2 hrs

Yellow color (+)

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Routine Testing • Quality Indicator

Enterobacteriaceae
Alternative method:
RAPID’Enterobacteriaceae

ISO 21528-2 (08/2004) Enumeration of

Enterobacteriaceae
Microbiology of food and animal NF VALIDATION according to Enterobacteriaceae
feeding stuffs ISO 16140
Horizontal methods for the detection N° BRD 07124-11/13
and enumeration of Enterobacteriaceae RAPID’Enterobacteriaceae

Non Enterobacteriaceae
Part 2: Colony-count technique

Ordering Information
Sample/initial suspension Sample/
initial suspension Catalog # Description

RAPID’Enterobacteriaceae/
Dilutions
Agar
Dilutions
Ready-to-use
VRBG 355-4012 200 ml x 6 bottles

RAPID’Enterobacteriaceae Dehydrated
(2 X)* 356-4004 500 g

Tryptone-Salt/Diluent
37°C Ready-to-use
24 hrs 355-5754 9 ml x 25 tubes
± 2 hrs 355-5756 90 ml x 6 bottles
355-5796 3 L x 4 bags
Reading 37°C**
± 1°C Dehydrated
24 hrs 356-4544 500 g
± 2 hrs

Confirmation Direct enumeration*

Nutrient (2.8%)
37°C
24 hrs
± 2 hrs
Biochemical confirmation

Oxidase (-) Dextrose BCP

37°C
24 hrs
± 2 hrs
Yellow color (+)
* Suitable with an automatic plate counter
** Alternatively, a temperature of 30°C or
* Please refer to ISO 7218: 2007 35°C can be chosen

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 Salmonella - ISO standard

EN ISO 6579/A1 (02/2006)

Microbiology of food and animal feeding stuffs

Horizontal method for the detection of Salmonella spp.

Amendment 1 Appendix D: Detection of Salmonella spp. in animal feces and in samples

from primary production stages

Sample
Non-selective
pre-enrichment

Buffered Peptone Water

Selective
37°C ± 1°C Appendix D Protocol
enrichment
18 hrs ± 2 hrs

Rappaport MKTTn MSRV

Vassiliadis Soya
37°C ± 1°C
41.5°C ± 1°C
24 hrs ± 3 hrs
24 hrs ± 3 hrs

41.5°C ± 1.5°C
Plating-out 24 hrs ± 3 hrs
(+24 hrs for negative)
Selective
isolation
XLD RAPID’Salmonella
or XLT 4
or Kristensen
or Hektoen
37°C ± 1°C
24 hrs ± 3 hrs

Confirmation 37°C ± 1°C

Nutrient 2.1% 24 hrs ± 3 hrs

Confirmation

Biochemical and serological confirmation

TSI Agar and other test

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Pathogen Testing

FDA-BAM Salmonella protocol FSIS Salmonella MLG 4.07

Sample Sample

Non-selective
pre-enrichment Non-selective
Pre-enrichment depends pre-enrichment
on the matrix

Lactose Broth Buffered Peptone Water

or Trypticase Soy Broth with/without Ferrous Sulfate or mTSB (for raw beef, carcass, and environmental samples)
or Universal Pre-enrichment Broth with/without Ferric
Ammonium Citrate
Selective 37°C
or Buffered Peptone Water enrichment 18-24 hrs
Depending on the
37°C food
Selective
enrichment 24 hrs ± 2 hrs Modified
Tetrathionate
Rappaport and
Broth
Rappaport Tretiationate Vassiliadis Broth
and
Vassiliadis Soya Broth 42°C
22-24 hrs
42°C 35°C or 43°C
24 hrs ± 2 hrs 24 hrs ± 2 hrs
Plating-out
Selective
isolation
Plating-out
Brillant Green XLT4 or
Selective and
Sulfat Agar DMLIA
isolation

XLD Hektoen Bismuth

Sulfite
35°C
18-24 hrs

37°C ± 1°C Confirmation

24 hrs ± 3 hrs

Confirmation

Biochemical and serological confirmation

TSI and LIA Agar

Biochemical and serological confirmation

TSI and LIA Agar

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 Salmonella - Alternative Salmonella - Alternative Salmonella - Alternative method:
method: RAPID’Salmonella Agar method: RAPID’Salmonella Agar iQ-Check Salmonella II
double enrichment protocol short protocol real-time PCR Kit

Detection of Salmonella spp. Detection of Salmonella spp. Detection of Salmonella spp.

NF VALIDATION according to Certification by NF VALIDATION NF VALIDATION according to ISO 16140
ISO 16140 according to ISO 16140 N° BRD 07/06-07/04
N° BRD 07/11-12/05 N° 07/11-12/05 AOAC-RI approved N° 010803*
NordVal approved N° 038
AOAC-RI approved N° 0507011* AOAC-RI approved N° 050701
Health Canada approved N° MFLP-38
All food stuffs and feed NordVal approved N° 032
All foods stuffs1,2, feed, and environment
including primary production

Sample Sample Sample

Buffered Peptone Water

Buffered 37°C
Peptone Water Buffered Peptone Water From 10 hrs ± 2 hrs
+ to 21 hrs ± 1 hr
Non-selective 37°C Salmonella Capsule Bacterial Lysis & DNA Extraction
pre-enrichment 18 hrs Supplement (Standard or Easy)
± 2 hrs

Rappaport Selective 41.5°C

Vassiliadis Soya enrichment ± 1°C
18 hrs Real-time PCR amplification and
41.5°C ± 2 hrs detection
Selective
enrichment 6-26 hrs with iQ-Check Salmonella II

Plating-out
Plating-out

RAPID’Salmonella
RAPID’Salmonella
Selective 37°C
Selective 37°C ±1°C Automatic result analysis
isolation
isolation 24 hrs 24 hrs
± 2 hrs
and interpretation
± 2 hrs
Reading & confirmation
Standard tests Reading & confirmation +/-
or Oxidase/OMNI-O/ONPG Salmonella Latex or Oxidase/
or iQ-Check Salmonella II OMNI-O/ONPG
or iQ-Check Salmonella Confirmation of positive with
RAPID’Salmonella
* Please refer to AOAC certificate for * Please refer to AOAC certificate for
validated matrices validated matrices

For more information on Bio-Rad’s real-time PCR kits and instruments, please
contact your local Bio-Rad sales representative.
1 - For AOAC-RI protocol, please contact your local Bio-Rad sales representative
2 - 8-hr enrichment protocol available for raw meat (AOAC and AFNOR validated)

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Pathogen Testing

Salmonella spp.

RAPID’Salmonella
Other
Enterobacteriaceae

Ordering Information
Catalog # Description

Antiserum Salmonella Novobiocin/Supplement Salmonella Confirm Latex Kit

Omnivalent OMNI-O (A60) Dehydrated 355-6711 50 tests
356-0781 3 ml dropper bottle 356-4610 1g
Selenite-Cystine/Broth
Brilliant Green (Kristensen)/Agar Nutrient (2.1%)/Agar Complete Medium
Dehydrated Dehydrated 355-5746 10 ml x 25 tubes
356-4464 500 g 356-4485 500 g
TSB/Broth
Buffered Peptone Water/Broth ONPG Test Ready-to-use
Ready-to-use 355-3822 Set of 50 disks (containing novobiocin)
355-4179 225 ml x 6 bottles 355-5426 225 ml x 6 bottles
355-5795 3 L x 4 bags Oxidase Test
355-5790 5 L x 2 bags 355-3834 2 x vials of Dehydrated Base
50 oxidase disks 356-4426 500 g
Dehydrated
356-4684 500 g RAPID’Salmonella/Agar TSI (Triple Sugar Iron)/Agar
Ready-to-use Dehydrated
Hektoen/Agar 356-3961 90 mm x 20 dishes 356-4384 500 g
Ready-to-use 356-3963 90 mm x 120 dishes
355-4386 100 ml x 6 bottles XLD (Xylose Lysine
356-3894 90 mm x 20 dishes Dehydrated Desoxycholate)/Agar
356-4705 500 g Ready-to-use
Dehydrated 354-1751 90 mm x 20 dishes
356-4284 500 g RAPID´Salmonella Capsules/
Supplement Dehydrated
iQ-Check Salmonella II Kit 356-4709 100 x quantity for 2.5 L 356-9124 500 g
357-8123 96 tests 356-4710 100 x quantity for 250 ml
356-4712 1 x quantity for XLT 4 (Xylose Lysine
MKTTN (Muller Kauffmann 100 analyses Tergitol 4)/Agar
Tetrathionate Novobiocin)/Broth Ready-to-use
Ready-to-use RVS/Broth 356-3654 90 mm x 20 dishes
355-6140 10 ml x 50 tubes (Rappaport Vassiliadis Soy)
Ready-to-use
Dehydrated Base 355-5773 10 ml x 25 tubes
356-4714 500 g
Dehydrated
MSRV (Semi-Solid Rappaport 356-4324 500 g
Vassiliadis Medium)
Salmonella Latex Kit
Ready-to-use
355-6139 200 ml x 6 bottles Detection of groups B to E and G
355-6710 75 tests
Dehydrated
356-4325 500 g

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 FDA BAM Cronobacter spp.
Cronobacter spp. - ISO standard protocol

ISO/TS 22964:2006 Samples

Milk and milk products -
Cronobacter spp.
Detection of Enterobacter
sakazakii Incubation
36°C ± 1°C
24 hrs ± 2 hrs
RAPID’Sakazakii
Other
Enterobacteriaceae 1:10
Buffered Peptone Water
Ordering Information
Sample preparation
Catalog # Description
Weigh z g of the test portion
Buffered Peptone Water/Broth in 9 x z ml Buffered Peptone
Ready-to-use Water
355-4179 225 ml x 6 bottles 40 ml
355-5795 3 L x 4 bags
355-5790 5 L x 2 bags 37°C
Dehydrated 18 hrs ± 2 hrs
356-4684 500 g

iQ-Check Cronobacter spp. Selective enrichment

357-8137 96 tests Transfer 0.1 ml in 10 ml
of mLST/vancomycin medium Centrifuge 3,000 x g for 10 min.
RAPID’Sakazakii/Agar Decant supernatant
Ready-to-use
356-3971 90 mm x 20 dishes
Resuspend pellet in 200 µl PBS
44°C
Dehydrated 24 hrs ± 2 hrs
356-4976 500 g

Trypto-Casein-Soy (TSA)/Agar
Ready-to-use
Streak onto RAPID’Sakazakii Spread and
356-3884 90 mm x 20 dishes streak plates
355-5947 200 ml x 6 bottles 100 µl
355-5944 8 ml x 25 inclined tubes 44°C
24 hrs ± 2 hrs DFI R&F
Agar Agar PCR PCR

Transfer 1 to 5 colonies on TSA

36°C ± 1°C
Incubation
18-24 hrs
25°C
48 hrs
Typical colonies from each plate
are confirmed with real-time PCR
Perform identification and other techniques
tests on yellow colonies

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Pathogen Testing

Cronobacter spp. - Alternative

method: RAPID’Sakazakii Agar Cronobacter spp. - Alternative method:
short protocol iQ-Check Cronobacter spp. real-time PCR Kit

RAPID’Sakazakii short protocol Detection of Cronobacter spp.

NF VALIDATION according to Infant Formulas and environment
ISO 16140 NF VALIDATION pending
N° BRD 07/22-05/12

Sample preparation Environmental sample Infant formula

Weigh z g of the test portion
in 9 x z ml BPW
Suppl. Buffered Peptone Water
Buffered Peptone Water
37°C 37°C
18 hrs ± 2 hrs 20 hrs ± 2 hrs
37°C
18 hrs ± 2 hrs Buffered Peptone Water
37°C
Streak onto RAPID’Sakazakii
4 hrs ± 1 hr

Bacterial Lysis & DNA Extraction

44°C
(Standard or Easy)
24 hrs ± 2 hrs

Real-time PCR amplification and detection

with iQ-Check Cronobacter spp.

Transfer 1 to 5 colonies on TSA

25°C
48-72 hrs

Perform identification
tests on yellow colonies Automatic result analysis and interpretation

+/-

Confirmation of positive with RAPID’Sakazakii

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 Listeria monocytogenes - ISO standards

EN ISO 11290-1/A1 (02/2005)

Horizontal method for the detection and enumeration of L. monocytogenes

Part 1: Detection method

Sample

Fraser ½
30°C
24 hrs ± 2 hrs
Primary enrichment
Isolation Secondary enrichment

Fraser 1
(0.1 ml culture/10 ml)
Agar Listeria according Other solid
to Ottaviani and Agosti selective medium 35°C or 37°C
48 hrs ± 2 hrs
37°C ± 1°C 30°C-35°C-37°C
24 hrs ± 3 hrs (depending Isolation
(+28 hrs ± 2 hrs)* of the media)
24-48 hrs ± 2 hrs Agar Listeria Other solid
Reading and according selective
confirmation to Ottaviani medium
and Agosti
37°C ± 1°C 30°C-35°C-37°C
24 hrs ± 3 hrs (depending on
Listeria spp. L. (+24 hrs ± 3 hrs) the media)
monocytogenes 24-48 hrs ± 2 hrs
- TSYEA agar
- Catalase + - TSYEB
- Gram test - Hemolysis Reading and
- Mobility - Carbohydrates confirmation
- CAMP test

* If low or absent colony growth observed after 24 hours.

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Pathogen Testing

Ordering Information
Catalog # Description
AL (Agar Listeria according
EN ISO 11290-2/A1 (02/2005)
to Ottaviani and Agosti)/Agar
Ready-to-use
Horizontal method for the detection and enumeration 356-3695 90 mm x 20 dishes
of L. monocytogenes 356-3965 90 mm x 120 dishes
Dehydrated Base
Part 2: Enumeration method 356-4043 500 g
Base in vials (to be supplemented)
355-5200 237.5 ml x 6 bottles
Supplement 1
356-4201 100 capsules
Sample (1 capsule qsp 250 ml)
356-4041 10 vials

Dilution Supplement 2
356-4042 10 vials

Buffered Peptone Water/Broth

Fraser ½ without LiCl Ready-to-use
and selective supplement 355-4179 225 ml x 6 bottles
or Buffered Peptone Water 355-5795 3 L x 4 bags
355-5790 5 L x 2 bags
Dehydrated
20°C ± 2°C
356-4684 500 g
1 hr ± 5 min
Fraser ½/Broth
Complete Medium
355-5797 225 ml x 6 bottles
Agar Listeria according 355-5794 3 L x 4 bags
to Ottaviani and Agosti 355-5792 5 L x 2 bags
Dehydrated Base
37°C 356-4604 500 g
24 hrs ± 3 hrs Supplement
(+ 24 hrs ± 3 hrs) 356-4616 10 vials
Enumeration and confirmation tests Fraser 1/Broth
Complete Medium
355-4569 10 ml x 25 tubes
Dehydrated Base
356-4604 500 g
Supplement
356-4615 10 vials
Listeria spp. Listeria Oxford/Agar
monocytogenes Ready-to-use
- TSYEA agar 356-3664 90 mm x 20 dishes
- Catalase + - TSYEB
- Gram test - Hemolysis Palcam/Agar
- Mobility - Carbohydrates Dehydrated Base
- CAMP test 356-4754 500 g
Supplement
356-4752 10 vials

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 Health Canada
Listeria monocytogenes
FDA-BAM Listeria monocytogenes protocol protocol MFHPB-30

Sample Sample

BLED
LEB
30°C
4 hrs
30°C 30°C 30°C
24 hrs 24 hrs 48 hrs
+ supplements

30°C 0.1 ml MFB 0.1 ml MFB

24 hrs ± 2 hrs (48 hrs ± 2 hrs)* Oxford + 35°C 35°C
RAPID’L.mono 24 hrs 24 hrs
(48 hrs) (48 hrs)
Agar Listeria
according to
Ottaviani and
Oxford Oxford + Oxford +
Agosti RAPID’L.mono
or RAPID’L.mono RAPID’L.mono
Palcam
Modified Oxford
(MOX) or and or

35°C
37°C 37°C 24 – 48 hrs
24 hrs (+24 hrs) 24 hrs (+24 hrs) 35°C 35°C
24 – 48 hrs 24 – 48 hrs

Reading, isolation, and confirmation

Real-time
PCR Reading and confirmation
amplification
and (optional)

- Motility - Real-Time PCR

Listeria spp. L. - Hemolysis Amplification
Real-time - Mannitol, rhamnose - Catalase
monocytogenes
- TSYEA agar PCR and xylose - Gram test
- Catalase + - TSYEB or amplification (or other equivalent - Serology
- Gram test - Hemolysis compendium-approved
- Mobility - Carbohydrates methods)
- CAMP test

* If low or absent colony growth observed after 24 hours

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Pathogen Testing

Listeria monocytogenes - Alternative method: RAPID’L.mono Agar

Detection of Listeria monocytogenes Enumeration of Listeria monocytogenes

NF VALIDATION according to ISO 16140 NF VALIDATION according to
N° BRD 07/4-09/98 ISO 16140
AOAC-RI approved N° 0304061* N° BRD 07/05-09/01
NordVal approved All food stuffs and environment
All food stuffs and environment

Sample Sample

Resuscitation

Fraser ½ Broth Buffered

Peptone Water
30°C
24 hrs
20°C
± 2 hrs
1 hr

Isolation on
RAPID’L.mono
Isolation on
37°C RAPID’L.mono
24 hrs
± 2 hrs 37°C
24 hrs
Reading and ± 2 hrs
confirmation tests
Direct
enumeration

Standard Tests
Spot on
Agar Listeria iQ-Check For a NF VALIDATION validated
- TSYEB or according to or Listeria or Rhamnose enumeration method, in the event
- Hemolysis Test
Ottaviani and monocytogenes II of a positive result using
- Carbohydrates
Agosti RAPID’L.mono for enumeration,
- CAMP test
confirmation is not necessary when the
presence of Listeria monocytogenes
* Please refer to AOAC certificate for validated matrices has been confirmed during detection.

For more information on Bio-Rad’s real-time PCR kits and instruments, please contact your local Bio-Rad sales representative.
1-F or AOAC-RI protocol, please contact your local Bio-Rad sales representative

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 Listeria monocytogenes - Alternative method:
iQ-Check Listeria monocytogenes II real-time PCR Kit
L. mono Detection of Listeria monocytogenes
NF VALIDATION according to ISO 16140
N° BRD 07/10-04/05
AOAC-RI approved N° 010802*
L. ivanovii NordVal approved N° 037
All food stuffs and environment

RAPID’L.mono L. innocua

Sample
L. welshimeri

Ordering Information (1)

Listeria Special Broth (LSB) or (2)
Fraser ½ Broth
Catalog # Description
30°C
Buffered Peptone Water/Broth 23 hrs ± 1 hr (1)
Ready-to-use or 25 hrs ± 1 hr (2)
355-4179 225 ml x 6 bottles
Bacterial Lysis & DNA Extraction
355-5795 3 L x 4 bags
355-5790 5 L x 2 bags Standard (Fraser ½ and LSB) or Easy (LSB)
Dehydrated
356-4684 500 g
iQ-Check
Listeria monocytogenes II Kit Real-time PCR amplification and detection with
357-8124 96 tests iQ-Check Listeria monocytogenes II
LSB (Listeria Special Broth)
Ready-to-use
355-5703 225 ml x 6 bottles
355-5793 5 L x 2 bags
Dehydrated
356-4703 500 g
356-4753 5 kg
RAPID’L.mono/Agar
Ready-to-use
356-3964 90 mm x 120 dishes Automatic result analysis and interpretation
356-3694 90 mm x 20 dishes
355-5294 Kit: 190 ml agar + 2 suppl.
Dehydrated Base
356-4293 500 g
+/-
Supplement 1
356-4294 10 vials
Confirmation of positive with RAPID’L.mono
Supplement 2
356-4746 10 vials
Rhamnose Test * Please refer to AOAC certificate for validated matrices
355-3669 28 tests

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Pathogen Testing

Listeria spp.
Alternative method:
RAPID’L.mono Agar Listeria spp. - Alternative method: RAPID’Listeria spp. Agar

Detection of Listeria spp. Detection of Listeria spp.

NF VALIDATION according to NF VALIDATION according to ISO 16140
ISO 16140 N° BRD 07/12-12/06
N° BRD 07/4-09/98 AOAC-RI approved N° 080701*
All food stuffs and environment All food stuffs and environment

Sample Sample

Fraser ½ Broth Fraser ½ Broth or UVM

30°C 30°C
24 hrs 24 hrs ± 2 hrs
± 2 hrs
Isolation on
RAPID’Listeria spp.

Isolation on
RAPID’L.mono

37°C 37°C
(24 hrs) 24 hrs ± 2 hrs
48 hrs

Reading and confirmation tests Reading and

confirmation tests

Standard Tests
- TSYEA or iQ-Check Standard Tests Spot on
- Gram test Listeria spp.
- Catalase or RAPID’L.mono or Spot on Palcam or iQ-Check
- Gram test + Gram test Listeria spp.
- Catalase + Catalase

* Please refer to AOAC certificate for validated matrices

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 Listeria spp. - Alternative method:
iQ-Check Listeria spp. real-time PCR Kit
Ordering Information
Detection of Listeria spp.
NF VALIDATION according to ISO 16140 Catalog # Description
N° BRD 07/13-05/07
Fraser ½/Broth
AOAC-RI approved N° 090701*
Ready-to-use
Health Canada Validation - MFLP-39 355-5797 225 ml x 6 bottles
All food stuffs and environment 355-5794 3 L x 4 bags
355-5792 5 L x 2 bags

Dehydrated Base
356-4604 500 g

Sample Supplement
356-4616 10 vials

iQ-Check Listeria spp. Kit

357-8113 96 tests
Listeria Special Broth (LSB)
LSB (Listeria Special Broth)
30°C Ready-to-use
23 hrs ± 1 hr (1) 355-5703 225 ml x 6 bottles
or 25 hrs ± 1 hr (2) 355-5793 5 L x 2 bags
Bacterial Lysis & DNA Extraction Dehydrated
((1) Standard or (2) Easy) 356-4703 500 g
356-4753 5 kg

RAPID’Listeria spp./Agar
Dehydrated Base
Real-time PCR amplification and detection with 356-4744 500 g
iQ-Check Listeria spp.
Supplement 1
356-4745 10 vials

Supplement 2

356-4746 10 vials

RAPID’L.mono/Agar
Dehydrated Base
356-4293 500 g

Automatic result analysis and interpretation Supplement 1

356-4294 10 vials (1 vial qsp 500 ml)

Supplement 2
+/- 356-4746 10 vials (1 vial qsp 500 ml)

For more information on Bio-Rad’s real-time PCR kits and

instruments, please contact your local Bio-Rad sales representative.
Confirmation of positive with RAPID’Listeria spp. or RAPID’L.mono *F
 or AOAC-RI protocol, please contact your local Bio-Rad sales
representative.

* Please refer to AOAC and Health Canada certificates for validated matrices

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Pathogen Testing

Escherichia coli O157:H7 - Alternative method:

RAPID’E.coli O157:H7 Agar

Detection of Escherichia coli O157:H7

NF VALIDATION according to ISO 16140
N° BRD 07/14-09/07
AOAC-RI approved N° 060701*
All food stuffs and environment
RAPID’E.coli O157:H7 E. coli O157:H7 Other E. coli

Ordering Information
Catalog # Description
Sample
Buffered Peptone Water/Broth
Ready-to-use
355-4179 225 ml x 6 bottles
355-5795 3 L x 4 bags
355-5790 5 L x 2 bags
mTSB + Novobiocin
Dehydrated
356-4684 500 g 41°C
iQ-Check E.coli O157:H7 Kit 16-24 hrs
357-8114 96 tests

iQ-Check STEC SerO Immunomagnetic-separation

357-8140 32 tests

iQ-Check STEC VirX

357-8139 96 tests

mTSB/Broth Isolation on RAPID’E.coli O157:H7

Ready-to-use
(containing novobiocin)
355-5426 225 ml x 6 bottles

Base
356-4426 500 g

Novobiocin/Supplement 37°C
Dehydrated 24 hrs ± 2 hrs
356-4610 1g

RAPID’E.coli O157:H7/Agar Reading and confirmation tests

Base Standard tests or Latex or
356-4748 100 g iQ-Check E.coli O157:H7
SEB (STEC Enrichment Broth)/Broth
356-4001 500 g
356-4002 5 kg

For more information on Bio-Rad’s real-time PCR kits and

instruments, please contact your local Bio-Rad sales representative. RAPID’E.coli O157:H7 can also be used as a second medium in the ISO 16654 standard
* Please refer to AOAC certificate for validated matrices

* For AOAC-RI protocol, please contact your local Bio-Rad sales representative.

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 STEC
iQ-Check STEC VirX and SerO Escherichia coli O157:H7 - Alternative method:
real-time PCR Kits iQ-Check E.coli O157:H7 real-time PCR Kit

Detection and Isolation Detection of Escherichia coli O157:H7

of non-O157 Shiga Toxin-Producing NF VALIDATION according to ISO 16140 N° BRD 07/15-06/08
E. coli (STEC) from ground beef Raw beef
AOAC-RI approved N° 020801*
USDA No Objection Letter Raw ground beef, fresh spinash, apple cider

Sample Sample

SEB enrichment
Buffered Peptone Water
41.5°C
10-15 hrs 41.5°C
8-24 hrs
Bacterial Lysis & DNA Extraction
Bacterial Lysis &
Easy DNA Extraction
Real-time PCR amplification and
detection with
iQ-Check STEC VirX
(Automatic results analysis and
Real-time PCR amplification and detection
interpretation)
with iQ-Check E.coli O157:H7

Real-time PCR amplification and

detection with
iQ-Check STEC SerO
(Automatic results analysis and
interpretation)

Result analysis and interpretation

+/-
+/-
Confirmation of posiitve with RAPID’E.coli O157:H7

* For AOAC-RI protocol, please contact your local Bio-Rad sales representative.

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Pathogen Testing

Campylobacter spp. - ISO standard

ISO 10272-1:2006 ISO/TS 10272-2:2006

Microbiology of food and animal feeding stuffs Microbiology of food and animal feeding stuffs
Horizontal method for detection and enumeration Horizontal method for detection and enumeration
of Campylobacter spp. of Campylobacter spp.

Part 1: Detection method Part 2: Enumeration method

Sample Sample

Dilution

Bolton Broth Isolation 0.1 ml

37°C + 41.5°C (microaerobic mCDD (x2)
4-6 hrs 44 ± 4 hrs atmosphere)
41.5°C (microaerobic
40-48 hrs atmosphere)
Innoculate a steril loop

Select 5 colonies

mCDD Agar Other solid

selective medium Isolation Columbia blood Agar
41.5°C (microaerobic 41.5°C
44 ± 4 hrs atmosphere) 24-48 hrs

Reading and confirmation

Isolation Columbia blood Agar

41.5°C
24-48 hrs Examination of morphology and motility

Reading and confirmation Study of growth 25°C (microaerobic

Columbia blood Agar 44 ± 4 hrs atmosphere)
Study of growth 41.5°C (aerobic
Columbia blood Agar 44 ± 4 hrs atmosphere)
Examination of morphology and motility
Oxidase
Study of growth 25°C (microaerobic
Columbia blood Agar 44 ± 4 hrs atmosphere)
Study of growth 41.5°C (aerobic
Columbia blood Agar 44 ± 4 hrs atmosphere)
Oxidase

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 Campylobacter spp. - Alternative method:
FDA-BAM Campylobacter spp. protocol iQ-Check Campylobacter spp. real-time PCR Kit

Most samples Detection of Campylobacter jejuni, Campylobacter coli

(except: shellfish, milk, frozen dairy products) and Campylobacter lari
AOAC-RI approved N° 031209*

For chicken carcass rinses, turkey swabs

and raw ground chicken
25 g sample (50 g vegetables)

100 ml Bolton Broth with antibiotics in net-lined bag Food Sample Carcass rinse or sponge
Suppl. Bolton Broth 2X BF-BEB
37°C ± 1°C 41.5°C ± 1°C 42°C
+
4 – 6 hrs 20 – 24 hrs 24 hrs
Rinse gently; shake 5 min. (25 rpm on bench-top shaker)
Remove net-lined bag

Bacterial Lysis and DNA Extraction

Preenrichment (Easy)

Microaerobic or Microaerobic
Except: bubbling at 30°C) Real-time PCR amplification and detection
37°C 37°C with iQ-Check Campylobacter
4 hrs 4 hrs

Microaerobic Enrichment
42°C
20-44 hrs

Streak 24 and 48 hrs enrichments on isolation agar

Result analysis and interpretation

Abeyta-Hunt-Bark agar or mCCDA

Incubate +/-
37°C or 42°C*
24-72 hrs
* in microaerobic
Identification

26 | | Food Safety and Quality Diagnostics • Bio-Rad Solutions * For AOAC-RI protocol, please contact your local Bio-Rad sales representative.

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Pathogen Testing

Campylobacter spp. - Alternative method: RAPID’Campylobacter spp.

Detection of Enumeration of
Campylobacter spp. Campylobacter spp.

NF VALIDATION according to
ISO 16140 N° BRD 07/25–01/14
RAPID’Campylobacter Campylobacter spp.

Ordering Information
Catalog # Description
Sample enrichment Suspension/Dilution
Buffered Peptone Water/Broth
Ready-to-use
355-4179 225 ml x 6 bottles
355-5795 3 L x 4 bags
355-5790 5 L x 2 bags
Preston Broth Dehydrated
356-4684 500 g
41.5 °C ± 1°C
Confirmation
24 hrs ± 2 hrs
Isolation on 356-3784 Columbia + Sheep
RAPID’Campylobacter Blood/Agar,
90 mm x 20 dishes
355-4164 Physiologic Sterile Water
(0.85%), 9 ml x 25 tubes
(microaerobic 41.5 °C ± 1°C 355-3834 Oxidase test,
atmosphere) (24 hrs) 2 x 50 disks
Isolation on 48 hrs 355-4154 Sterile Distilled Water,
RAPID’Campylobacter 9 ml x 25 tubes
Reading and confirmation tests
356-4297  Campylobacter Confirm
Campylobacter Confirm Latex Latex kit, 50 tests
(microaerobic 41.5 °C ± 1°C
atmosphere) 24-48 hrs or
iQ-Check Campylobacter iQ-Check Campylobacter/Kit
or 357-8135 96 tests
Reading and confirmation tests Standard tests RAPID’Campylobacter/Agar
Campylobacter Confirm Latex Dehydrated Base
or 356-4295 500 g
iQ-Check Campylobacter
Supplement
or
356-4296 10 vials (1 vial qsp 400 ml)
Standard tests
Tryptone Salt/Diluent
Ready-to-use
355-5756 90 ml x 6 bottles
355-5754 9 ml x 25 tubes
355-5747 9 ml x 100 tubes
355-5796 3 L x 4 bags

Dehydrated
356-4544 500 g

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 Staphylococcus aureus - ISO standards

NF EN ISO 6888-1/A1 (01/2004) NF EN ISO 6888-2/A1 (12/2003) NF EN ISO 6888-3 (06/2003)

Horizontal method for the Horizontal method for the Horizontal method for the
enumeration of coagulase-positive enumeration of coagulase-positive enumeration wof coagulase-positive
Staphylococci (S. aureus Staphylococci (S. aureus Staphylococci (S. aureus
and other species) and species) and species)
Technique including confirmation Technique using rabbit plasma Detection and MPN technique
of colonies fibrinogen agar medium for low numbers

Sample/initial suspension Sample/initial suspension Sample/initial suspension

and following dilution and following dilution

Giolitti and Cantoni Giolitti and Cantoni

Broth (or)* Broth
Baird-Parker Simple concentration Double concentration
Baird-Parker 37°C
for RPF 24 hrs ± 2 hrs
(2 X)*
(+24 hrs ± 2 hrs)*
+ RPF sup.
35°C or 37°C
24 hrs ± 2 hrs
(2 X)*
(+ 24 hrs ± 2 hrs)**
Enumeration Baird-Parker Baird-Parker
35°C or 37°C
or for RPF
18-24 hrs
+ RPF sup.
and 48 hrs 37°C 37°C
Confirmation 24-48 hrs 24-48 hrs
Brain-Heart Infusion Direct enumeration of typical
coagulase-positive Staphylococci Confirmation of Direct
35°C or 37°C
24 hrs ± 2 hrs typical colonies enumeration
on BHI of typical
Rabbit Plasma coagulase-
positive colonies
35°C or 37°C
4-6 hrs or 24 hrs 37°C
24 hrs
Coagulation reading
Rabbit Plasma
Typical Staphylococci 37°C
grey to black colonies 4-6 hrs
with a surrounding clear halo or 24 hrs
and a white opaque zone Reading
* Please refer to ISO 7218: 2007 * For detection method only
** If low or absent colony growth observed * If low or absent colony growth observed
after 24 hours. * Please refer to ISO 7218: 2007 after 24 hours.

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Pathogen Testing

Staphylococcus aureus
FDA BAM Alternative method:
Staphylococcus aureus RAPID’Staph Agar
Ordering Information
Sample Enumeration of Coagulase-positive
Staphylococci Catalog # Description
NF VALIDATION according to ISO 16140
Dilutions Additives
N° BRD 07/9-02/05
Egg Yolk with Potassium Tellurite
AOAC-RI approved N° 080602* 355-4201 5 ml x 1 vial
All food stuffs, feed and environment 355-4205 25 ml x 1 bottle
Spread 1 ml on 3 plates
Baird Parker Sulfamethazine Solution (0.2%)
356-2682 2.5 ml x 1 vial

Baird-Parker/Agar
Sample/initial suspension Complete Medium
356-3991 90 mm x 20 dishes

Base
35°C 356-4814 500 g
45-48 hrs
Baird-Parker for RPF/Agar
Complete Medium
Confirmation 357-8618 90 ml x 6 bottles
+ 6 vials of suppl.
RAPID’Staph 356-3996 90 mm x 20 dishes
37°C
Dehydrated Base
24 hrs ± 2 hrs
356-4814 500 g
Enumeration and Confirmation RPF Supplement
356-4618 10 vials

BHI (Brain Heart Infusion)/Broth

Ready-to-use
355-3664 10 ml x 25 tubes
Spot on Dehydrated
Pastorex™
or Baird-Parker 356-4014 500 g
Staph Plus
for RPF
Pastorex Staph Plus Latex
™

355-6356 50 tests
355-6353 5 x 50 tests

Rabbit Plasma
Freeze-dried
355-6352 Pack for 20 reactions

Special broth
355-3544 9 ml x 25 tubes

RAPID’Staph/Agar
Ready-to-use
356-3960 90 mm x 20 dishes
* For AOAC-RI protocol, please contact
your local Bio-Rad sales representative
Base
* Please refer to AOAC certificate for
validated matrices 356-4704 500 g

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 Sulfite-reducing bacteria Clostridium perfringens

ISO 15213 (05/2003) NF EN ISO 7937 (02/2005)

Microbiology of food and animal Horizontal method for

feeding stuffs the enumeration of
Horizontal method for the Clostridium perfringens
TSC Clostridium perfringens enumeration of sulfite-reducing
bacteria growing under anaerobic Colony-count technique
conditions
Ordering Information
Catalog # Description
Food sample/initial suspension Food sample/initial suspension
Lactose Sulfite/Broth
Base Heat
356-4914 100 g treatment
20 min 75°C
Sheep Blood/Agar TSC
Ready to use Dilution (add D-cycloserine)
356-3784 90 mm x 20 dishes

Dehydrated Base (2 X)*

356-4674 500 g

Sheep Blood Supplement TSC Anaerobes 37°C

355-6652 50 ml vial condition 20 hrs ± 2 hrs
Thioglycolate + Resazurin/Broth
Ready to use (2 X)* Enumeration
356-3613 10 ml x 25 tubes of typical black colonies
Confirmation
Dehydrated of 5 isolated colonies with
Thermophilous 37°C
356-4084 500 g
bacteria 24-48 hrs Thioglycolate + Resazurin
TSC without D-Cycloserine/Agar - suspicion anaerobic
50°C 24-48 hrs
Anaerobes 37°C
Base Medium condition
condition 18-24 hrs
Ready to use
355-4419 100 ml x 6 bottles Reading
Base
356-9644 500 g
Lactose Mobility Lactose
Sulfite Nitrate Gelatine
Confirmation 46°C 37°C
24 hrs 18-24 hrs

Clostridium Clostridium Clostridium

perfringens perfringens perfringens
(black (red (yellow/
precipitate/ coloration/ gas+)
gas+) gas+)

* Please refer to ISO 7218: 2007 * Please refer to ISO 7218: 2007

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Pathogen Testing

Bacillus cereus Shigella

NF EN ISO 7932 (07/2005) NF EN ISO 21567 (03/2005)

General guidance for the Horizontal method

enumeration of B. cereus for the detection of Shigella spp.

Colony-count technique at 30°C Mossel (MYP) Bacillus cereus

Ordering Information
Catalog # Description
Food sample/initial suspension Food sample/initial suspension
Hektoen/Agar
Ready-to-use
355-4386 100 ml x 6 bottles
356-3894 90 mm x 20 dishes
Mossel (MYP) Shigella Broth + Novobiocin
Dehydrated
pH 7 ± 0.2 356-4284 500 g
41°C Mac Conkey + Crystal Violet/Agar
(2 X)* 16-20 hrs Ready-to-use
356-3617 90 mm x 20 dishes
Plating-out
30°C Dehydrated
18-24 hrs 356-9084 500 g
(+24 hrs)
Novobiocin/Supplement
Dehydrated
Confirmation test Mac XLD Hektoen 356-4610 1g
on 5 well isolated colonies Conkey
Nutrient (2.1%)/Agar
Dehydrated
356-4485 500 g

Mossel/Agar
Sheep Blood/Agar 37°C (Mannitol Egg Yolk Polymixin)
20-24 hrs Dehydrated
30°C (+48 hrs)* 356-9604 500 g
24 hrs ± 2 hrs
XLD (Xylose Lysine
Spot on typical colonies on
Desoxycholate)/Agar
Reading and interpretation Nutrient (2.1%) Ready-to-use
354-1751 90 mm x 20 dishes
37°C
20-24 hrs Dehydrated
356-9124 500 g
Biochemical and serological
confirmation

* If low or absent colony growth observed

* Please refer to ISO 7218: 2007 after 24 hours.

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Validations

PCR Methods Chromogenic Methods

iQ-Check Solutions RAPID’ Solutions
NF Health NF Health
AOAC NordVal AOAC NordVal
Validation Canada Validation Canada

Campylobacter spp.

Coliforms
(pending)

Cronobacter spp.

Escherichia coli
(pending

Escherichia coli O157:H7

Enterobacteriaceae

Detection
Listeria (pending
monocytogenes
Enumeration

Listeria spp.

Salmonella Enteritidis

Salmonella spp.

Salmonella Typhimurium

Staphylococcus aureus

STEC 7 major O-groups*

STEC virulence genes*

* No objection letter delivered from USDA

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Bio-Rad Solutions

Range for Food Testing

Meat Water
Solution Segment Dairy Environment Processed Vegetables
& Poultry & & Wine
Microorganism Products Foods Produce
Fish Products Beverages

Bacillus cereus

Campylobacter

Clostridium perfringens

Coliforms

Cronobacter sakazakii

Enterobacteriaceae

Escherichia coli

Escherichia coli O157:H7

STEC

Lactobacillus

Listeria spp.

Listeria monocytogenes

Pseudomonas aeruginosa

Salmonella spp.

Salmonella Enteritidis

Salmonella Typhimurium

Staphyloccocus aureus

Yeasts and Molds

RAPID’Chromogenic method iQ-Check PCR method Classical media

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 Real-Time PCR Methods • Food Testing

Start, Run, Results

iQ-Check™ Prep Solution, when the power

of automation meets real-time PCR
• Reliable walk-away automation for real-time PCR testing
• Designed to fit lab workflow without disrupting it
• Increased confidence with improved reproducibility
• Optimized to handle all major food pathogens

For more information: foodscience.bio-rad.com

iQ-Check Prep, CFX96 Touch™ deep well and iQ-Check Kits

The Bio-Rad PCR Solution, Your Solution

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Bio-Rad
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