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Lab Report For Cape Unit 1 Biology Investigative Project

1. The document describes an experiment that compares the effectiveness of three different washing powders - Rosey, Sud Sud, and ADA - at removing a soy sauce stain from cloth. 2. It finds that ADA, which contains the protease enzyme, was most effective and removed the stain in 14 hours and 38 minutes. Rosey, containing laccases, took 15 hours and 13 minutes. Sud Sud, containing no enzymes, took the longest at 16 hours and 45 minutes. 3. The conclusion is that enzymes help break down proteins and lipids in stains, and the presence of protease in ADA allows it to most quickly remove the soy sauce stain due to protease breaking down the protein in soy sauce.

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Emily Zheng
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100% found this document useful (1 vote)
657 views3 pages

Lab Report For Cape Unit 1 Biology Investigative Project

1. The document describes an experiment that compares the effectiveness of three different washing powders - Rosey, Sud Sud, and ADA - at removing a soy sauce stain from cloth. 2. It finds that ADA, which contains the protease enzyme, was most effective and removed the stain in 14 hours and 38 minutes. Rosey, containing laccases, took 15 hours and 13 minutes. Sud Sud, containing no enzymes, took the longest at 16 hours and 45 minutes. 3. The conclusion is that enzymes help break down proteins and lipids in stains, and the presence of protease in ADA allows it to most quickly remove the soy sauce stain due to protease breaking down the protein in soy sauce.

Uploaded by

Emily Zheng
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Date: Mar.

24th, 2021

Title: Effectiveness of enzymic washing powder

Aim: To compare the rate of stain removal using different enzymic washing powders

Apparatus: a timer, a triple beam balance, three beakers, a measuring cylinder, a dropper, three
pieces plain cloth

Materials: two different types of enzymic washing powders, one enzyme-free washing powder,
distilled water, soy sauce

Method:
v 30g of each type of washing powders were measured and each portion was placed into a
beaker.
v 50ml of distilled water was measured and poured into each beaker and was mixed
thoroughly with each portion of washing powder.
v 3ml of soy sauce was measured and a dropper was used to drop 1ml of it on each plain
cloth.
v Each piece of plain cloth was waited to dry.
v The stained cloth was placed inside the soap powder solution.
v A timer was used to record the time taken for each stain to disappear.
v Cloths were lined up for comparison.

Data collected:

C (ADA) B (SudSud) A (Rosey)


TABLE SHOWING TYPES OF WASHING POWDER, ENZYME CONTAINED AND THE
TIME TAKEN TO REMOVE THE SOY SAUCE STAIN ON EACH CLOTH
Types of washing powder Enzyme(s) contained Time taken the remove the stain (s)

Washing powder A (Rosey) Bioactive blue enzymes(laccases) 15 hours and 13 minutes

Washing powder B (Sud Sud) No enzyme 16 hours and 45 minutes

Washing powder C (ADA) Protease 14 hours and 38 minutes

Discussion:
Enzyme, a substance that acts as a catalyst in living organisms, regulating the rate at chemical
reactions proceed without itself being altered in process. In this experiment, the stain is soy sauce
which contains high protein (from soybeans) and lipids. The washing powder A (Rosey) contains
bioactive blue enzyme laccases catalyzes the oxidation reaction for various aromatic compounds
and the reduction reaction for oxygen to form water took 15 hours and 13 minutes to remove the
stain. It took a longer time to remove the stain compare to washing powder C because the laccases
in washing powder A only break down lipids in soy sauce, which is not rich in soy sauce. The
control in this experiment is Washing powder B (Sud Sud) took the longest time (16 hours and 45
minutes) to remove the soy sauce stain because it does not have any enzyme to break down
protein. The washing powder C (ADA) contains an enzyme called protease that breaks down
protein(main ingredients from the soy sauce stain) into amino acids which is soluble in water.
Washing powder C took the shortest time to remove the soy sauce stain among the three washing
powders used. Among three experiment, the cloth submerged in washing powder C was the
whitest and the cleanest for washing powder C has protease that break down high content of
protein in soy sauce and some trace of stain can be found on the cloth submerged in washing
powder B for Sud Sud does not contain any enzyme to break down neither protein nor lipids.

Limitations:
Each teaspoon of different washing powders may not have the same number of enzymes, e.g
washing powder A has 300 enzymes in a teaspoon and washing powder C has 260 enzymes in a
teaspoon.
Systematic error on beam balance specifically zero error.
Precautions:
Ensure that the mass of each soap powder is the same.
Ensure that the volumes of water and soy sauce used for each portion are the same.

Ensure that all measurements are read at eye level.

Reflection:
This lab helped us in considering the right enzymatic washing powder based on the satin present
and reduce the water usage/waste in the environment when washing because the faster the
enzymes remove the stains, the less time it would take to wash thus saving the water. This lab also
bettered our understanding of enzyme concept in a real life situation.

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