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03 05 Cellular Respiration Lab Report

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119 views

03 05 Cellular Respiration Lab Report

Uploaded by

maddisonlooker
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Cellular Respiration Lab Report

Instructions: In this laboratory activity, you will calculate the rate of cellular respiration in
germinating and non-germinating peas using a respirometer to measure the amount of oxygen
consumed. You will also investigate the effects of temperature, light, or seed type on the rate of
respiration. Submit your lab report to your instructor when completed.

Title:

Objective(s):

Hypothesis:

There are two parts of this lab activity. This means you should have two hypotheses. In part one,
you are testing the rate of cellular respiration of germinating and non-germinating seeds. In part
two, you will select a different factor to test, such as temperature, light, or seed type, and
measure the effects of this factor on the rate of respiration. Write each hypothesis using an
if/then statement.

Procedure:

Access the virtual lab and complete the electrophoresis procedure. The materials are listed for
you. However, you are responsible for providing a brief summary of the steps you followed
during the experimental procedure.

Materials:

Cellular Respiration Virtual Lab

Summary of Steps:

Variables:

List your controlled variables, independent variable, and dependent variable of each part of the
lab activity. Explain why these are the variables.

Remember, controlled variables are factors that remain the same throughout the experiment. An
independent (test) variable changes so that the experimenter can see the effect on other variables.
The dependent (outcome) variable will change in response to the test variable.
Controlled variables:

Independent variable:

Dependent variable:

Data:

Complete the data tables to organize the data collected in this lab. Don’t forget to record
measurements with the correct number of significant figures.

Important: Before you record your volume, you must correct for differences in volume
that are due to temperature fluctuation rather than rate of respiration. To do this, subtract
any difference in the movement of water into the vial with glass beads from the
experimental vials held at the same temperature. For example, if the five-minute reading
measures 3 ml for the germinating peas, but the glass beads (which are not respiring)
show an increase of 1 ml, subtract 1 ml from 3 ml and record the total volume as 2 ml.

Table 1

This table is for gathering data on the respiration of germinating and non-germinating seeds.

Respirometer 1 Respirometer 2 Respirometer 3


Total Time (min) Volume of water (ml) Volume of water (ml) Volume of water (ml)
0
5
10
15
20
25

Table 2

This table is for gathering data on the effects of temperature, light, or seed type on respiration.

Respirometer 1 Respirometer 2 Respirometer 3 Respirometer 4


Total time (min) Volume of Water (ml) Volume of Water (ml) Volume of Water (ml) Volume of Water (ml)
0
5
10
15
20
25
Graphing

Create a graph to compare the rate of respiration of the respirometers 2 and 4 in Table 2 of your
investigation. The graph should show the change in the volume versus time. This represents the
rate of respiration. Be sure to include a graph title and properly label your x- and y-axes,
including units.

Conclusion:

Write a conclusion statement that addresses the following questions:

 How was the rate of cellular respiration affected by germinating and non-germinating
peas? How was the rate affected by temperature, light, or seed type?
 Does your data support or fail to support your hypotheses (include examples)?
 Discuss the sources of error that could impact the results of this investigation if it were
repeated in a physical laboratory.

Lab Reflection Questions

Answer the reflection questions, using what you have learned from the lesson and your
experimental data. It will be helpful to refer to your class notes. Answer questions in complete
sentences.

1. Explain how germinating peas use cellular respiration to obtain energy stored within their
endosperm?
2. If germinating seeds could only use fermentation to obtain energy, what changes would
you expect in the rate of respiration and growth?

3. Statistical analysis allows scientists to evaluate the reliability of experimental data in


order to support or refute the null or alternate hypothesis of an investigation. One
standard deviation on either side of the mean represents 68% confidence that the true
value lies within that interval. (For the questions below, refer to your pre-lab activity for
assistance.)

a) Calculate the mean respiration rate of your germinating peas and non-
germinating peas. Before you take the mean of all 6 trials you must change your
volume/5 mins into volume/1 min. Divide each interval reading by 5 to get an
average per minute rate. Calculate one standard deviation for both means.
b) Construct a bar graph that shows the mean respiration rate for germinating peas
and the mean respiration rate for non-germinating peas. Add error bars to show a
68% confidence interval across each mean.
c) Is there evidence in your respiration data and your bar graph to support the claim
that most respiration rates lie within one standard deviation of the mean?

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