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LncRNA

de novo LncRNA prediction

Checking the quality of RNA-seq and filter low quality reads

Aligning RNA-seq to reference genome with Tophat2

Assembly and quanlitation of transcripts with Cufflinks

Discovering known and de novo LncRNAs

Differential expression analysis for LncRNAs with Cufflinks

Making plots with R

reference:

  1. Hu B, Huo Y, Chen G, Yang L, Wu D, Zhou J: Functional prediction of differentially expressed lncRNAs in HSV-1 infected human foreskin fibroblasts. Virol J 2016, 13:137.
  2. Hu B, Huo Y, Yang L, Chen G, Luo M, Yang J, Zhou J: ZIKV infection effects changes in gene splicing, isoform composition and lncRNA expression in human neural progenitor cells. Virol J 2017, 14(1):217.

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de novo LncRNA prediction

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